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Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates

Niknejad, F. and Moshfegh, M. and Najafzadeh, M.J. and Houbraken, J. and Rezaei, S. and Zarrini, G. and Faramarzi, M.A. and Nafissi-Varcheh, N. (2013) Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates. Iranian Journal of Pharmaceutical Research, 12 (SUPPL.). pp. 111-117. ISSN 17350328 (ISSN)

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Abstract

Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isolate U3; CBS 132822, and isolate U4; CBS 132823). The growth of these isolates was determined by measuring the colony diameter and mycelia dry weight in Sabouraud dextrose agar and yeast nitrogen base medium supplemented with NaCl, KCl, and LiCl. Isolate U4 showed a growth up in 15% NaCl and U1 was the only isolate that could grow in 20% KCl. None of the strains grew in a media containing LiCl. The salt supplemented medium did not increase the size of colony diameter in all isolates (p > 0.05). The ability of the selected isolates for amylase production was quantitatively tested and showed that P. polonicum isolate U4 was the most potent producer of amylase with a yield of 260.9 U/L after 60 h, whereas P. polonicum isolate U3 was the lowest one with a production level of 97.9 U/L after 48 h. P. polonicum isolate U4 could be a suitable candidate for production of amylase on an industrial scale after optimization. © 2013 by School of Pharmacy.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Pharm. Res. [Field not mapped to EPrints] AD - Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Pharmaceutical Biotechnology, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, and Cancer Molecular Pathology Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran [Field not mapped to EPrints] AD - Department of Applied and Industrial Mycology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands [Field not mapped to EPrints] AD - Department of Animal Sciences, University of Tabriz, Tabriz, Iran [Field not mapped to EPrints] AD - Department of Pharmaceutical Biotechnology, School of Pharmacy and Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Amylase, Fungi, Halotolerant, Hypersaline environments, Isolation, alpha tubulin, amylase, amylase activity, article, fungus, fungus growth, fungus isolate, nonhuman, Penicillium, Penicillium chrysogenum, Penicillium polonicum, phenotype, phylogeny, salt tolerance
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:54
Last Modified: 10 May 2015 10:10
URI: http://eprints.goums.ac.ir/id/eprint/1899

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