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Effect of maternal morphine sulfate exposure on neuronal plasticity of dentate gyrus in Balb/c mice offspring

Golalipour, M.J. and Ghafari, S. and Kaboli Kafshgiri, S. and Latifi Moghadam, M.H. and Moharri, A.R. (2013) Effect of maternal morphine sulfate exposure on neuronal plasticity of dentate gyrus in Balb/c mice offspring. Pakistan Journal of Biological Sciences, 16 (6). pp. 281-286. ISSN 10288880 (ISSN)

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Abstract

This study carried out to evaluate the effects of maternal morphine exposure during gestational and lactation period on the neuronal cells of dentate gyrus in 18 and 32 days Balb/c mice offspring. In this experimental study 10 female mice were randomly allocated into cases and controls. In experimental group, animals were received morphine sulfate 10 mg/kg/body weight intraperitoneally during 7 days before mating, gestational period (GD0-21), 18 and 32 days after delivery. The control animals were received an equivalent volume normal saline. Cerebrum of six infant for each group were removed and stained with cresyl violet and monoclonal anti-neuronal nuclei (NeuN) antibody. Quantitative computer-assisted morphometric study was done on dentate gyrus of hippocampus. In the P18 mice, the numbers of granular cells in dentate gyrus medial blade and dentate gyrus lateral blade significantly reduced from 171.45±4.2 and 174.51±3.1 cells in control group to 153.32±2.8 and 151.23±3.2 cells in 10000 μm2 area of granular layer in treated group (p<0.001). In P32 mice the numbers of granular cells in mb and lb of dentate gyrus significantly decreased from 155.31±4.1 and 153.77±3.4 in control group to 138.33±4.5 and 135.13±4.3 in treated group, respectively (p<0.001). The granular layer thickness in mb and lb area of dentate gyrus significantly reduced in treated mice in compared to controls in P18 and P32 mice (p<0.05). This study revealed that morphine administration before, during pregnancy and lactation period causes neuronal cells loss of dentate gyrus in 18 and 32 days old infant mice. © 2013 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Pak. J. Biol. Sci. [Field not mapped to EPrints] C2 - 24498791 [Field not mapped to EPrints] AD - Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Histology laboratory, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Balb/c mouse, Dentate gyrus, Granular cells, Morphine sulfate, Prenatal exposure, morphine, narcotic analgesic agent, age, animal, article, Bagg albino mouse, cell death, dentate gyrus, drug effect, environmental exposure, female, gestational age, lactation, mouse, nerve cell, nerve cell plasticity, newborn, pathology, pregnancy, prenatal exposure, Age Factors, Analgesics, Opioid, Animals, Animals, Newborn, Cell Death, Dentate Gyrus, Female, Gestational Age, Lactation, Maternal Exposure, Mice, Mice, Inbred BALB C, Morphine, Neuronal Plasticity, Neurons, Pregnancy, Prenatal Exposure Delayed Effects, Animalia, Mus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 21 Apr 2015 04:23
Last Modified: 20 Jun 2015 04:57
URI: http://eprints.goums.ac.ir/id/eprint/1896

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