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Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major

Ghaffarifar, F. and Jorjani, O. and Sharifi, Z. and Dalimi, A. and Hassan, Z.M. and Tabatabaie, F. and Khoshzaban, F. and Hezarjaribi, H.Z. (2013) Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major. APMIS, 121 (4). pp. 290-298. ISSN 09034641 (ISSN)

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Abstract

Leishmaniasis is an important disease in humans. Leishmania homologue of receptor for Activated C Kinase (LACK) and thiol specific antioxidant (TSA) as immuno-dominant antigens of Leishmania major are considered the most promising molecules for a DNA vaccine. We constructed a DNA cocktail, containing plasmids encoding LACK and TSA genes of Leishmania major and evaluated the immune response and survival rate in BALB/c mice. IgG and Interferon gamma values were noticeably increased in the immunized group with DNA cocktail vaccine, which were significantly higher than those in the single-gene vaccinated and control groups (p < 0.05) following the immunization and after challenging with Leishmania major. Interleukin 4 values were decreased in all immunized groups, but only in DNA vaccine cocktail and single-gene vaccination with pc-LACK there were statistical differences with control groups (p > 0.05). The immunized mice with the cocktail DNA vaccine presented a considerable reduction in diameter of lesion compared to other groups and a significant difference was observed (p < 0.05) in this regard. The survival time of the immunized mice with the cocktail DNA vaccine was significantly higher than that in the other groups (p < 0.05) after their being challenged with Leishmania major. The findings of this study indicated that the cocktail DNA vaccine increased the cellular response and survival rate and induced protection against infection with Leishmania in the mice. © 2012 APMIS.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - APMIS [Field not mapped to EPrints] C2 - 23030526 [Field not mapped to EPrints] AD - Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Biotechnology, Faculty of Advanced Medical Technology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran [Field not mapped to EPrints] AD - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Parasitology, Faculty of Medical Sciences, Tehran Medical Science University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Parasitology, Faculty of Medical Sciences, Shahed University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cocktail pcTSA+pcLACK, DNA vaccine, Immune responses, Leishmania major, DNA vaccine, gamma interferon, immunoglobulin G, interleukin 4, animal cell, animal experiment, animal model, article, controlled study, gene, immune response, immunization, leishmania homologue of receptor for activated c kinase gene, Leishmania major, leishmaniasis, mouse, nonhuman, nucleotide sequence, priority journal, spleen cell, survival rate, thiol specific antioxidant gene, Animals, Antibodies, Protozoan, Antigens, Protozoan, Female, Immunization, Immunoglobulin G, Leishmania major, Leishmaniasis Vaccines, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Peroxiredoxins, Protozoan Proteins, Vaccines, DNA
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 04:25
Last Modified: 17 Jan 2017 10:14
URI: http://eprints.goums.ac.ir/id/eprint/1895

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