Golestan University of Medical Sciences Repository

Interleukin-12 as a genetic adjuvant enhances hepatitis C virus NS3 DNA vaccine immunogenicity

Naderi, M. and Saeedi, A. and Moradi, A. and Kleshadi, M. and Zolfaghari, M.R. and Gorji, A. and Ghaemi, A. (2013) Interleukin-12 as a genetic adjuvant enhances hepatitis C virus NS3 DNA vaccine immunogenicity. Virologica Sinica, 28 (3). pp. 167-173. ISSN 16740769 (ISSN)

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Abstract

Hepatitis C virus (HCV) chronic infection is a worldwide health problem, and numerous efforts have been invested to develop novel vaccines. An efficient vaccine requires broad immune response induction against viral proteins. To achieve this goal, we constructed a DNA vaccine expressing nonstructural 3 (NS3) gene (pcDNA3.1-HCV-NS3) and assessed the immune response in C57BL/6 mice. In this study, the NS3 gene was amplified with a nested-reverse transcriptase-polymerase chain reaction (RT-PCR) method using sera of HCV-infected patients with genotype 1a. The resulting NS3 gene was subcloned into a pcDNA3.1 eukaryotic expression vector, and gene expression was detected by western blot. The resultant DNA vaccine was co-administered with interleukin-12 (IL-12) as an adjuvant to female C57BL/6 mice. After the final immunizations, lymphocyte proliferation, cytotoxicity, and cytokine levels were assessed to measure immune responses. Our data suggest that co-administration of HCV NS3 DNA vaccine with IL-12 induces production of significant levels of both IL-4 and interferon (IFN)-γ (p<0.05). Cytotoxicity and lymphocyte proliferation responses of vaccinated mice were significantly increased compared to control (p<0.05). Collectively, our results demonstrated that co-administration of HCV NS3 and IL-12 displayed strong immunogenicity in a murine model. © 2013 Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Virol. Sin. [Field not mapped to EPrints] C2 - 23709057 [Field not mapped to EPrints] AD - Department of Microbiology, Qom Branch, Islamic Azad University, Qom 37185-364, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan 1616, Iran [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Department of Microbiology, Golestan University of Medical Sciences, Gorgan 4917765181, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research Centre, Tehran 1996835911, Iran [Field not mapped to EPrints] AD - Institut für Physiologie i, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse, Münster 48149, Germany [Field not mapped to EPrints] AD - Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität Münster, Münster 48149, Germany [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: DNA vaccine, Hepatitis C virus (HCV), Interleukin-12, NS3, DNA vaccine, gamma interferon, hepatitis C vaccine, immunological adjuvant, interleukin 12, interleukin 4, nonstructural protein 3, virus DNA, animal cell, animal experiment, animal model, animal tissue, article, cellular immunity, controlled study, cytokine production, cytokine response, cytotoxic T lymphocyte, cytotoxicity, female, gene expression profiling, genotype, hepatitis C, Hepatitis C virus, human, immunogenicity, lymphocyte proliferation, molecular cloning, mouse, nonhuman, NS3 gene, protein induction, reverse transcription polymerase chain reaction, serology, vaccination, Western blotting, Adjuvants, Immunologic, Animals, Cell Proliferation, Cytokines, Cytotoxicity, Immunologic, Female, Interleukin-12, Leukocytes, Mononuclear, Mice, Mice, Inbred C57BL, Vaccines, DNA, Viral Nonstructural Proteins, Viral Vaccines, Eukaryota, Hepatitis C virus, Murinae, Mus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:03
Last Modified: 20 Jun 2015 04:54
URI: http://eprints.goums.ac.ir/id/eprint/1885

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