Golestan University of Medical Sciences Repository

Preventing effect of vitamin E on oocytes apoptosis in morphinetreated mice

Asadi, E. and Shabani, R. and Ghafari, S. and Golalipour, M.J. (2013) Preventing effect of vitamin E on oocytes apoptosis in morphinetreated mice. International Journal of Morphology, 31 (2). pp. 533-538. ISSN 07179367 (ISSN)

[img]
Preview
PDF - Published Version
Download (94kB) | Preview

Abstract

Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10 Units) and 10 Unites of HCG. The next day the animals were sacrificed, oocytes were flushed from each fallopian tube. The collected oocytes were subjected to determine apoptosis by Tunnel assay with using Fluorescent Microscope. According to our results, the number of retrieved oocytes were 121, 132, 86 and 114 in control, experimental group I, II and III, respectively. Morphine Sulfate treatment increased apoptosis in oocytes to 17.44% whereas oocytes apoptosis was 4.13% in Controls. Supplementation with Vitamin E in Morphine Sulfate -treated mice reduced the oocytes apoptosis to 7.01%. This study showed that Morphine can increase apoptosis in oocytes and Vitamin E treatment significantly reduces oocytes apoptosis in the Morphine Sulfate -treated mice.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English; Spanish [Field not mapped to EPrints] ST - Efecto preventivo de la vitamina E sobre la apoptosis de ovocitos en ratones tratados con morfina [Field not mapped to EPrints] J2 - Int. J. Morphol. [Field not mapped to EPrints] AD - Department of Anatomical sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Anatomical sciences, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Gorgan Congenital Malformations Research Center, Department of Anatomical sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Apoptosis, Mice, Morphine sulfate, Oocytes, TUNEL assay, Vitamin E
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 05:13
Last Modified: 02 Jul 2015 05:55
URI: http://eprints.goums.ac.ir/id/eprint/1881

Actions (login required)

View Item View Item