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Effect of IL-22 on DNA vaccine encoding LACK gene of Leishmania major in BALB/c mice

Hezarjaribi, H.Z. and Ghaffarifar, F. and Dalimi, A. and Sharifi, Z. and Jorjani, O. (2013) Effect of IL-22 on DNA vaccine encoding LACK gene of Leishmania major in BALB/c mice. Experimental Parasitology, 134 (3). pp. 341-348. ISSN 00144894 (ISSN)

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In the present study, the effect of IL-22 together with the plasmid encoding LACK (Leishmania homolog of receptors for activated C-kinase) gene of Leishmania major on the trend of leishmaniasis in BALB/c mice was evaluated.Evaluation of the cellular and humoral immunity was performed by measurement of IL-4 and IFN-γ, culture of splenocytes and MTT assay, and measurement of total IgG, IgG1, and IgG2a in the control and immunized groups. Clinical evaluations were also carried out by measurement of the lesion size, survival rate, and body weight of mice.Comparison of the mean size of lesions in the LACK and LACK. +. IL-22 groups demonstrated that the mean size of lesions of the two groups was significantly different from week four (p<. 0.05).The survival rate at day 170 after challenge for the PBS, pcDNA3 (empty plasmid), pcLACK (pcDNA3 containing LACK gene), and pcLACK. +. IL-22 groups were 20%, 40%, 60%, and 80%, respectively.According to the results of IFN-γ, IL-4, total IgG, IgG1, and IgG2a measurement and the MTT assay, IL-22 obviously caused an increase in IFN-γ production and a decrease in IL-4 production before and after the challenge (p<. 0.05). The results showed the effectiveness of IL-22 in DNA vaccine. It showed that IL-22 brought about Th1 cytokine responses and high survival rate of mice. © 2013 Elsevier Inc.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Exp. Parasitol. [Field not mapped to EPrints] C2 - 23541883 [Field not mapped to EPrints] AD - Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: DNA vaccine, IL-22, Leishmania major, PcLACK, 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, DNA vaccine, gamma interferon, immunoglobulin G antibody, immunoglobulin G1 antibody, immunoglobulin G2a antibody, interleukin 22, interleukin 4, animal cell, animal experiment, animal model, article, body weight, cellular immunity, controlled study, drug effect, drug efficacy, female, gene, humoral immunity, Leishmania homolog of receptor for activated C kinase gene, leishmaniasis, mouse, nonhuman, priority journal, promastigote, survival rate, Th1 cell, vaccination, Animals, Antibodies, Protozoan, Antigens, Protozoan, Body Weight, Female, Immunoglobulin G, Interferon-gamma, Interleukin-4, Interleukins, Leishmania major, Leishmaniasis, Cutaneous, Lymphocytes, Mice, Mice, Inbred BALB C, Protozoan Proteins, Protozoan Vaccines, Spleen, Survival Rate, Vaccines, DNA
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:15
Last Modified: 26 Feb 2018 06:45
URI: http://eprints.goums.ac.ir/id/eprint/1863

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