Golestan University of Medical Sciences Repository

Spectroscopic and microscopic analyses of rod-shaped gold nanoparticles interacting with single-stranded DNA oligonucleotides

Saber, R. and Shakoori, Z. and Sarkar, S. and Tavoosidana, G. and Kharrazi, S. and Gill, P. (2013) Spectroscopic and microscopic analyses of rod-shaped gold nanoparticles interacting with single-stranded DNA oligonucleotides. IET Nanobiotechnology, 7 (2). pp. 42-49. ISSN 17518741 (ISSN)

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Abstract

The application of rod-shaped gold nanoparticles as probes and carriers in biological systems have recently attracted great interest. UV-vis spectroscopy, circular dichroism, Fourier transform infrared spectroscopy, scanning electron microscopy and atomic force microscopy were used to study optical and structural properties of rod-shaped gold nanoparticles when interacting with DNA oligomers in phosphate sodium salt buffer. The morphological transformation process of rod-shaped gold nanoparticles resulting from the interaction with single-stranded DNA (ssDNA) showed the role of hexadecyltrimethylammonium bromide (CTAB) in nanostructures as the main interacting agent. The obtained results confirmed that the CTAB coat of rod-shaped gold nanoparticles have powerful positive charges for conjugations with surface negative charges of phosphate groups on ssDNA oligomers. The CTAB also inhibit the formation of covalent sulphide bonds between the gold core of rod-shaped nanoparticles and alkanethiol oligonucleotides. The authors found that when the nanorods were exposed to ssDNA oligonucleotides, the gold nanorods changed their shapes and sizes, and exposed some microscopic malformations which could be used in the development of colorimetric assays of nucleic acids. © The Institution of Engineering and Technology 2013.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - IET Nanobiotechnol. [Field not mapped to EPrints] C2 - 24046904 [Field not mapped to EPrints] AD - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), P.O. Box 1978647653, Tehran, Iran [Field not mapped to EPrints] AD - Nanotechnology Group, Research Center for Science and Technology in Medicine (RCSTIM), Tehran University of Medical Sciences (TUMS), P.O. Box 1978647653, Tehran, Iran [Field not mapped to EPrints] AD - Department of Medical Nanotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences (GOUMS), P.O. Box 1673978964, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Colorimetric assays, Gold Nanoparticles, Hexadecyltrimethylammonium bromide, Interacting agents, Microscopic analysis, Morphological transformations, Single-stranded DNA, UV-vis spectroscopy, Atomic force microscopy, DNA, Fourier transform infrared spectroscopy, Metal nanoparticles, Nanorods, Oligomers, Oligonucleotides, Scanning electron microscopy, Ultraviolet visible spectroscopy, Gold, gold, metal nanoparticle, nanotube, oligonucleotide, single stranded DNA, article, atomic force microscopy, chemistry, circular dichroism, infrared spectroscopy, transmission electron microscopy, ultrastructure, ultraviolet spectrophotometry, Circular Dichroism, DNA, Single-Stranded, Gold, Metal Nanoparticles, Microscopy, Atomic Force, Microscopy, Electron, Transmission, Nanotubes, Oligonucleotides, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:50
Last Modified: 20 Jun 2015 04:35
URI: http://eprints.goums.ac.ir/id/eprint/1862

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