Golestan University of Medical Sciences Repository

Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model

Yazdani, Y. and Keyhanvar, N. and Kalhor, H.R. and Rezaei, A. (2013) Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model. Biotechnology Letters, 35 (8). pp. 1191-1197. ISSN 01415492 (ISSN)

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Abstract

Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01). © 2013 Springer Science+Business Media Dordrecht.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Biotechnol. Lett. [Field not mapped to EPrints] C2 - 23553521 [Field not mapped to EPrints] AD - Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Biochemistry Research Laboratory, Department of Chemistry, Sharif University of Technology, Tehran, Iran [Field not mapped to EPrints] AD - Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Baculovirus expression system (BES), Ferroportin, Hepcidin, Iron metabolism, Baculovirus expression systems, Cell culture media, Ferroportin, Hepcidin, Iron concentrations, Iron metabolism, Peritoneal macrophage, Protease inhibitor, Cardiovascular system, Iron, Mammals, Metabolism, Cell culture, cation transport protein, Hamp1 protein, mouse, hepcidin, iron, metal transporting protein 1, recombinant protein, animal, animal model, article, biosynthesis, blood, cell line, drug effect, genetics, macrophage, metabolism, mouse, Animals, Cation Transport Proteins, Cell Line, Hepcidins, Iron, Macrophages, Mice, Models, Animal, Recombinant Proteins, Animalia
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 10:05
Last Modified: 11 May 2015 06:03
URI: http://eprints.goums.ac.ir/id/eprint/1848

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