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The effect of Ginkgo biloba extract on scopolamine-induced apoptosis in the hippocampus of rats

Jahanshahi, M. and Nickmahzar, E.G. and Babakordi, F. (2013) The effect of Ginkgo biloba extract on scopolamine-induced apoptosis in the hippocampus of rats. Anatomical Science International, 88 (4). pp. 217-222. ISSN 14476959 (ISSN)

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Abstract

Apoptosis, known as programmed cell death, plays a crucial role in normal development and tissue homeostasis. Apoptosis is also involved in neurodegenerative diseases such as Alzheimer's disease. Amnesia refers to the loss of memory and can also be a warning sign of neurodegenerative diseases. The antioxidant properties of Ginkgo biloba extract was known previously. Therefore, the aim of this study was to examine the effects of Ginkgo biloba extract on the rat's hippocampal apoptotic neurons number after Scopolamine based amnesia. Thirty-six adult male Wistar rats were used. Rats were randomly divided into control, sham, protective and treatment groups. The rats in the sham group received only scopolamine hydrobromide (3 mg/kg) intraperitoneally. The rats in the protective and treatment groups received Ginkgo biloba extract (40, 80 mg/kg) for 7 days intraperitoneally before/after scopolamine injection. Then 48 h after the last injection, the brains of rats were withdrawn and fixed with paraformaldehyde, and then, after histological processing, the slices were stained with the TUNEL kit for apoptotic neurons. Data were compared by the ANOVA Post Hoc Tukey test; P < 0.05 was considered significant. Our results showed that Scopolamine (in the sham group) increased significantly the number of apoptotic neurons in all areas of the hippocampus compared with the control. Whereas, Ginkgo biloba extract reduce the neuronal apoptosis in the hippocampus before and/or after encounter with scopolamine. We concluded that pretreatment and treatment injection of Ginkgo biloba extract can have a protective effect for neurons and it can limit apoptosis in all area of the hippocampus. © 2013 Japanese Association of Anatomists.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Anat. Sci. Int. [Field not mapped to EPrints] C2 - 23828103 [Field not mapped to EPrints] AD - Department of Anatomy, Faculty of Medicine, Neuroscience Research Center, Golestan University of Medical Sciences, 4 Gorgan-Sari Road (Shastcola), Gorgan, Iran [Field not mapped to EPrints] AD - Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Apoptosis, Ginkgo biloba extract, Hippocampus, Rat, Scopolamine, Ginkgo biloba extract, paraformaldehyde, scopolamine bromide, neuroprotective agent, plant extract, scopolamine bromide, amnesia, animal cell, animal experiment, article, brain protection, brain slice, cell protection, controlled study, drug effect, hippocampus, histology, male, nerve cell necrosis, nonhuman, priority journal, rat, animal, apoptosis, chemically induced disorder, cytology, Ginkgo biloba, hippocampus, nerve cell, pathology, Wistar rat, Amnesia, Animals, Apoptosis, Ginkgo biloba, Hippocampus, Male, Neurons, Neuroprotective Agents, Plant Extracts, Rats, Rats, Wistar, Scopolamine Hydrobromide
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 08:53
Last Modified: 10 May 2015 06:14
URI: http://eprints.goums.ac.ir/id/eprint/1839

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