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Cytochrome c oxidase subunit 1 and 12S ribosomal RNA characterization of Coenurus cerebralis from sheep in Iran

Rostami, S. and Salavati, R. and Beech, R.N. and Sharbatkhori, M. and Babaei, Z. and Saedi, S. and Harandi, M.F. (2013) Cytochrome c oxidase subunit 1 and 12S ribosomal RNA characterization of Coenurus cerebralis from sheep in Iran. Veterinary Parasitology, 197 (1-2). pp. 141-151. ISSN 03044017 (ISSN)

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Abstract

Taenia multiceps is a widely distributed zoonotic tapeworm of canids. The larval stage of the parasite (Coenurus) occurs in sheep, goat and cattle and has been rarely reported from humans. This study investigated genetic variability of two mitochondrial genes in 102 isolates of T. multiceps.Metacestodes were collected from brains and hearts of sheep in Tehran and Qom provinces of Iran. DNA of each isolate was extracted and used for PCR amplification of cytochrome c oxidase subunit I (CO1) and 12S ribosomal DNA (12S rRNA) genes. All amplicons were sequenced and the sequence data were analyzed using NCBI Blast and BioEdit. Phylogenetic trees and pairwise calculations were obtained by using Mega5 software.In total 7 and 25 representative haplotypes were differentiated for CO1 and 12S rRNA genes, respectively. For CO1 sequences 11 segregation sites within 7 haplotypes were observed. For 12S rRNA sequences a total of 32 segregation sites were observed in 25 haplotypes. CO1 gene displayed lower diversity than 12S rRNA gene with an overall nucleotide variation of 3.0% for CO1 vs. 7.2% for 12S rRNA. Pairwise comparisons among 7 haplotypes in CO1 and 12S rRNA genes showed the level of nucleotide differences 0.3-2.5% and 0.2-4.0%, respectively.A high degree of genetic variation was found in the isolates of T. multiceps in Iran. Additional molecular studies are required on the parasite from other intermediate hosts. © 2013 Elsevier B.V.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Vet. Parasitol. [Field not mapped to EPrints] C2 - 23890823 [Field not mapped to EPrints] AD - Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman 76169-14111, Iran [Field not mapped to EPrints] AD - Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Shahid Rajaee Hospital, Alborz University of Medical Sciences, Karaj, Iran [Field not mapped to EPrints] AD - Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, 76169-14111, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Genetic variation, Iran, Mitochondrial DNA, Sheep, Taenia multiceps, cytochrome c oxidase, genomic DNA, mitochondrial DNA, RNA 12S, 12S rRNA gene, animal tissue, article, coenurosis, COI gene, controlled study, gene, gene segregation, gene sequence, genetic analysis, genetic variability, haplotype, host parasite interaction, Iran, metacestode, nonhuman, nucleotide sequence, parasite identification, parasite isolation, phylogenetic tree, polymerase chain reaction, RNA sequence, sheep, Taenia multiceps, Bos, Canidae, Capra hircus, Ovis aries, Taenia multiceps, Genetic variation, Iran, Mitochondrial DNA, Sheep, Taenia multiceps, Animals, Base Sequence, Cestoda, Cestode Infections, DNA, Helminth, Electron Transport Complex IV, Genetic Variation, Iran, Protein Subunits, RNA, Ribosomal, Sheep, Sheep Diseases
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:55
Last Modified: 10 May 2015 15:01
URI: http://eprints.goums.ac.ir/id/eprint/1824

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