Golestan University of Medical Sciences Repository

Scopolamine reduces the density of M1 muscarinic neurons in rats' hippocampus

Jahanshahi, M. and Nickmahzar, E.G. and Seif-Hoseini, S. and Babakordi, F. and Moharreri, A. (2013) Scopolamine reduces the density of M1 muscarinic neurons in rats' hippocampus. International Journal of Morphology, 31 (4). pp. 1227-1232. ISSN 07179367 (ISSN)

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Abstract

Cholinergic system in CNS is involved in learning and memory. Scopolamine as muscarinic acetylcholine receptor antagonist is used for creation of memory impairment. The purpose of this study is evaluation of scopolamine-based amnesia on memory retention and the effect of this phenomenon on the number of neurons contains M1-receptors in the male Wistar rats hippocampal regions. Thirty-five male Wistar rats (200±20 g) were distributed randomly into five groups. Control group (intact samples) and 3 experimental groups with sham group (saline) were tested by the method of passive avoidance (shuttle box) in doses of 0.2, 0.5 and 1 mg/kg (intraperitoneally) as a single dose. After one week, memory test was taken from the rats. Finally, brains dissected from sacrificed rats, and then processed tissues were stained with antibody against M1 receptors (Immunohistochemistry technique) followed by counting of hippocampal CA1, CA3 and DG regions. Our results showed significant decrease in neurons contains M1-receptors in all area of hippocampus. We found that the less number of M1-neurons showed in 1 mg/kg dose of scopolamine. We concluded that scopolamine as muscarinic acetylcholine receptor antagonist can reduce dose-dependently the density of M1-neurons in all areas of hippocampus.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English; Spanish [Field not mapped to EPrints] ST - La escopolamina reduce la densidad de las neuronas muscarínicas M1 en el hipocampo de ratas [Field not mapped to EPrints] J2 - Int. J. Morphol. [Field not mapped to EPrints] AD - Neuroscience Research Center, Department of Anatomy, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Avoidance memory, Hippocampus, M1 receptor, Rat, Scopolamine
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 04:18
Last Modified: 10 May 2015 06:20
URI: http://eprints.goums.ac.ir/id/eprint/1810

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