Golestan University of Medical Sciences Repository

A nanodiagnostic colorimetric assay for 18S rRNA of leishmania pathogens using nucleic acid sequence-based amplification and gold nanorods

Niazi, A. and Jorjani, O.N. and Nikbakht, H. and Gill, P. (2013) A nanodiagnostic colorimetric assay for 18S rRNA of leishmania pathogens using nucleic acid sequence-based amplification and gold nanorods. Molecular Diagnosis and Therapy, 17 (6). pp. 363-370. ISSN 11771062 (ISSN)

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Abstract

Background and Objective: We describe here a nanodiagnostic colorimetric assay for 18S rRNA of Leishmania pathogens that uses nucleic acid sequence-based amplification (NASBA) and gold nanorods (GNRs). Methods: NASBA primers targeting 18S rRNA were used for amplification of RNA in an isothermal process. The electrostatic interactions between the phosphate groups of the RNA amplicons and the cetyl trimethylammonium bromide layer on the GNRs resulting in their aggregation. This phenomenon changes the color of the GNR solution from red to purple. Results: Our data showed 100 % sensitivity and 80 % specificity with the colorimetric assay compared with results using reverse transcription polymerase chain reaction. Conclusion: The nanodiagnostic method we describe simplifies the detection of NASBA amplicons without the need for gel electrophoresis. © 2013 Springer International Publishing Switzerland.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Mol. Diagn. Ther. [Field not mapped to EPrints] C2 - 23771698 [Field not mapped to EPrints] AD - Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Laboratory Sciences, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Nanotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: cetrimide, gold nanoparticle, gold nanorod, nanorod, phosphate, RNA 18S, unclassified drug, amplicon, article, clinical article, colorimetry, controlled study, human, Leishmania major, nonhuman, nucleic acid sequence based amplification, priority journal, reverse transcription polymerase chain reaction, sensitivity and specificity, static electricity, Colorimetry, DNA Primers, Gold, Humans, Leishmania, Leishmaniasis, Nanomedicine, Nanotubes, RNA, Protozoan, RNA, Ribosomal, 18S, Self-Sustained Sequence Replication, Sensitivity and Specificity
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 05:31
Last Modified: 20 Jun 2015 06:28
URI: http://eprints.goums.ac.ir/id/eprint/1805

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