Golestan University of Medical Sciences Repository

Enhanced cell immune responses to hepatitis c virus core by novel heterologous DNA prime/lambda nanoparticles boost in mice

Saeedi, A. and Ghaemi, A. and Tabarraei, A. and Moradi, A. and Gorji, A. and Semnani, S. and Soleimanjahi, H. and Adli, A.H. and Hosseini, S.Y. and Vakili, M.A. (2014) Enhanced cell immune responses to hepatitis c virus core by novel heterologous DNA prime/lambda nanoparticles boost in mice. Virus Genes, 49 (1). pp. 11-21. ISSN 09208569 (ISSN)

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Abstract

Hepatitis C virus (HCV) is a worldwide problem which does not have an effective vaccine and more than 170 million people worldwide are chronically infected by HCV. T cell responses are associated with spontaneous clearance of HCV infection. We report here the development of recombinant Lambda bacteriophage nanoparticles encoding HCV Core antigen. The aim of this study was to investigate the antigen-specific immune responses triggered in mice by different prime-boost combinations of DNA and Lambda phage nanoparticles encoding the HCV Core. The homologous prime/boost with recombinant Lambda nanoparticles induced higher levels of cellular and humoral immune response than the DNA vaccines. However, a heterologous prime/boost of HCV Core protein, using DNA vaccine priming followed by Lambda boost, induced highest level of lymphocyte proliferation, CD8 lymphocytes with cytotoxic function, and shifting the immune response toward a T helper (Th1) pattern and in overall improved immunity. Our study provides a new, safe, and effective vaccine for the prime-boost regimen which augments robust immunity and highlights novel promising strategies in HCV vaccine development. © 2014 Springer Science+Business Media.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Virus Genes [Field not mapped to EPrints] C2 - 24752903 [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research Centre, Tehran, Iran [Field not mapped to EPrints] AD - Institut für Physiologie I, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse, 48149 Munster, Germany [Field not mapped to EPrints] AD - Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany [Field not mapped to EPrints] AD - Department of Neurology, Westfälische Wilhelms-Universität Münster, Münster, Germany [Field not mapped to EPrints] AD - Department of Virology, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Shiraz University of Medical Sciences, Shiraz, Iran [Field not mapped to EPrints] AD - Department of Health and Social Medicine, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Antibody, Cellular Immunity, DNA vaccine, HCV Core gene, Lambda nanoparticles, Prime/boost, core protein, DNA vaccine, gamma interferon, hepatitis C antibody, hepatitis C vaccine, immunoglobulin G antibody, immunoglobulin G1 antibody, immunoglobulin G2c antibody, interleukin 4, nanoparticle, unclassified drug, core protein, DNA vaccine, drug carrier, hepatitis C antibody, nucleocapsid protein, Hepatitis C virus, recombinant vaccine, virus vaccine, animal cell, animal experiment, antibody response, article, bacteriophage lambda, CD8+ T lymphocyte, cell mediated cytotoxicity, cellular immunity, controlled study, cytotoxic T lymphocyte, Hepatitis C virus, humoral immunity, lymphocyte proliferation, mouse, nonhuman, priority journal, protein expression, Th1 cell, animal, blood, C57BL mouse, cell proliferation, Enterobacteria phage lambda, female, gene vector, genetics, Hepacivirus, immunology, procedures, vaccination, Hepatitis C virus, Mus, Animals, Bacteriophage lambda, CD8-Positive T-Lymphocytes, Cell Proliferation, Drug Carriers, Female, Genetic Vectors, Hepacivirus, Hepatitis C Antibodies, Mice, Inbred C57BL, Nanoparticles, Vaccination, Vaccines, DNA, Vaccines, Synthetic, Viral Core Proteins, Viral Vaccines
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:48
Last Modified: 23 May 2015 06:18
URI: http://eprints.goums.ac.ir/id/eprint/1798

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