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Allelic forms of merozoite surface protein-3 in Plasmodium falciparum isolates from southeast of Iran

Ebrahimzadeh, A. and Mohammadi, S. and Jamshidi, A. (2014) Allelic forms of merozoite surface protein-3 in Plasmodium falciparum isolates from southeast of Iran. Jundishapur Journal of Microbiology, 7 (5). ISSN 20083645 (ISSN)

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Abstract

Background: Genetic diversity has provided Plasmodium falciparum with the potential capacity of avoiding the immune response, and possibly supported the natural selection of drug or vaccine-resistant parasites. Merozoite surface protein-3 (MSP-3) has been used to develop vaccines and investigate the genetic diversity regarding P. falciparum malaria in Iran. Objectives: The main goal of this study was to analyze the polymorphic antigen MSP-3 genes across southeast of Iran among four different districts, to identify the differences in the allele frequency and genetic diversity. Materials and Methods: Nested polymerase chain reaction amplification was used to determine polymorphisms of N-terminal region of the MSP-3 gene. A total of 85 microscopically positive P. falciparum infected individuals from southeast of Iran were included in this study. Results: Of the 85 confirmed P. falciparum samples obtained from four different districts, 72 were successfully scored for MSP-3.The MSP-3 allele classes (K1 and 3D7 types) showed comparable prevalence in all districts. Overall frequencies of K1 and 3D7 allele classes were 94.5 % for both. Conclusions: Since no study has yet looked at the extent of P. falciparum MSP-3 in this geographic region, these data can be helpful to support development of a vaccine based on MSP-3 against malaria. There should be a comparative analysis in different seasonal peaks to indicate the allelic polymorphism of MSP-3 over a period. © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp.

Item Type: Article
Additional Information: Unmapped bibliographic data: C7 - e9829 [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - Jundishapur J. Microbiol. [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, Iran [Field not mapped to EPrints] AD - Department of Molecular Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Genetic variation, Iran, Merozoite surface protein 3, Plasmodium, Plasmodium falciparum, merozoite surface protein 3, parasite antigen, unclassified drug, allele, amino terminal sequence, article, controlled study, gene frequency, genetic polymorphism, genetic variability, human, Iran, major clinical study, malaria falciparum, microbial gene, MSP 3 gene, nonhuman, Plasmodium falciparum
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 04:12
Last Modified: 04 May 2015 09:21
URI: http://eprints.goums.ac.ir/id/eprint/1796

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