Golestan University of Medical Sciences Repository

Comparing the effect of Toll-like receptor agonist adjuvants on the efficiency of a DNA vaccine

Sajadian, A. and Tabarraei, A. and Soleimanjahi, H. and Fotouhi, F. and Gorji, A. and Ghaemi, A. (2014) Comparing the effect of Toll-like receptor agonist adjuvants on the efficiency of a DNA vaccine. Archives of Virology, 159 (8). pp. 1951-1960. ISSN 03048608 (ISSN)

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Abstract

We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were inoculated with 2 � 105 TC-1 cells, and they were then immunized with HPV-16 E7 DNA vaccine alone or with 50 μg of resiquimod or poly(I:C) individually. We found that poly(I:C) and resiquimod could induce more antigen-specific lymphocyte proliferation and cytolytic activity compared to vaccination with E7 DNA alone. While E7 DNA had no significant inhibitory effect on tumor growth, co-administration of poly(I:C) and resiquimod with E7 DNA induced significant tumor regression. Peripheral and local cytokine assays demonstrated that co-administration of poly(I:C) and resiquimod with E7 DNA induced circulating antigen-specific IFN-γ and nonspecific intratumoral IL-12. TLR3 and TLR7 agonists can be used to enhance the immune response to DNA vaccine immunogens. Taken together, these data indicate that combined vaccination with DNA encoding HPV-16 E7 plus TLR agonists provides a strategy for improving the efficacy of a vaccine as a possible immunotherapeutic strategy for cervical cancer. © 2014 Springer-Verlag Wien.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Arch. Virol. [Field not mapped to EPrints] C2 - 24573220 [Field not mapped to EPrints] AD - Shefa Neuroscience Research Center, Tehran, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Virology, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Virology, Pasteur Institute of Iran, Tehran, Iran [Field not mapped to EPrints] AD - Institut für Physiologie I, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse, Münster, Germany [Field not mapped to EPrints] AD - Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität Münster, Münster, Germany [Field not mapped to EPrints] AD - Department of neurology, Westfälische Wilhelms-Universität Münster, Münster, Germany [Field not mapped to EPrints] AD - Shefa Neuroscience Research Center, Khatam Al-Anbiya Hospital, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Human papillomavirus, Human papillomavirus type 16, Mus, DNA vaccine, immunological adjuvant, oncogene protein E7, Human papillomavirus type 16, protein E7, toll like receptor 3, toll like receptor 7, Wart virus vaccine, agonists, animal, C57BL mouse, comparative study, female, genetics, human, Human papillomavirus type 16, immunology, Uterine Cervical Neoplasms, vaccination, virology, Adjuvants, Immunologic, Animals, Female, Human papillomavirus 16, Humans, Mice, Inbred C57BL, Papillomavirus E7 Proteins, Papillomavirus Vaccines, Toll-Like Receptor 3, Toll-Like Receptor 7, Uterine Cervical Neoplasms, Vaccination, Vaccines, DNA
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:09
Last Modified: 09 May 2015 05:31
URI: http://eprints.goums.ac.ir/id/eprint/1790

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