Golestan University of Medical Sciences Repository

Allelic variation of polymorphic vaccine candidates merozoite surface protein-2 in Plasmodium falciparum isolates from south-east of Iran

Namdar, F. and Ebrahimzadeh, A. and Mohammadi, S. (2014) Allelic variation of polymorphic vaccine candidates merozoite surface protein-2 in Plasmodium falciparum isolates from south-east of Iran. Advances in Environmental Biology, 8 (1). pp. 155-158. ISSN 19950756 (ISSN)

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Abstract

Background: Genetic diversity of Plasmodium falciparum-the main causative agent of malaria- provides the parasitewith the potential of escaping the immune response and results in the selection of vaccine and drug-resistant species.Study the allelic variation of different vaccine candidate genes in regions of malaria territorycould be used to design and introducenewtherapeutic methods. Therefore, Merozoite surface protein 2 (MSP-2) was selected for the purpose of evaluating allelic variation in the southeastern region of Iran. Materials and Methods: In this study Nested Polymerase Chain Reaction (Nested-PCR) amplification was used to determine different allelic forms of MSP-2 gene using specific oligonucleotides. A total of 94 microscopically positive P. falciparum infected individuals from South-East of Iran were included. Findings: Of all94 Plasmodium falciparum specimens, 85wereconfirmed for the presence of MSP-2alleles. The frequency of MSP-2 different allelic classes was considerably high and calculated to be 50.5% and 34.2 % for 3D7 and FC 27 respectively. Conclusion: Both dimorphic alleles of MSP-2 gene were detected where the frequency of 3D7 was the highest among the regions.The frequency of the alleles does not differ much from the results of studies in other regions of the world. However, this information can be beneficial to have a new vaccine designed on the basis of studies on the candidateantigens. © 2014 AENSI Publisher All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Adv. Environ. Biol. [Field not mapped to EPrints] AD - Department of Parasitology, Zahedan University of Medical Sciences, Zahedan, Iran [Field not mapped to EPrints] AD - GolestanUniversity of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Allelic variation, Glutamine rich protein, Iran, Merozoite surface protein 2, Plasmodium falciparum
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 08:46
Last Modified: 15 Jun 2015 06:52
URI: http://eprints.goums.ac.ir/id/eprint/1789

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