Golestan University of Medical Sciences Repository

The comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria

Shafipour, M. and Ghane, M. and Rahimi alang, S. and Livani, S. and Ghaemi, E. (2014) The comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria. Archives of Razi Institute, 69 (2). pp. 137-142. ISSN 03653439 (ISSN)

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Abstract

The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the most prevalent region of tuberculosis in Iran. Among 3336 patients suspected to tuberculosis referred to hospitals and health care centres in Golestan province during 2010-2011, 319 (9.56%) culture positive cases were collected. Identification of species by using biochemical tests was done. On the samples recognized as nontuberculous Mycobacteria, after DNA extraction by boiling, 16S rDNA PCR was done and their sequencing were identified by NCBI BLAST. Of the 319 positive samples in Golestan Province, 300 cases were M.tuberculosis and 19 cases (5.01%) were identified as nontuberculous Mycobacteria by biochemical tests. 15 out of 19 nontuberculous Mycobacteria were identified by PCR and sequencing method as similar by biochemical methods (similarity rate: 78.9%). But after PCR, 1 case known as M.simiae by biochemical test was identified as M. lentiflavum and 3 other cases were identified as Nocardia. Biochemical methods corresponded to the 16S rDNA PCR and sequencing in 78.9% of cases. However, in identification of M. lentiflavum and Nocaria sp. the molecular method is better than biochemical methods. © 2014 by Razi Vaccine & Serum Research Institute.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Arch. Razi Inst. [Field not mapped to EPrints] AD - Young Researchers Club, Islamic Azad University of TonekabonTonekabon, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Islamic Azad University of TonekabonTonekabon, Iran [Field not mapped to EPrints] AD - Laboratory of Tuberculosis, Health Care Centre of Golestan Province, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Infectious Research Centre and Microbiology Department, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 16S rDNA, M. lentiflavum, M. simiae, Nontuberculous mycobacteria, Corynebacterineae
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:08
Last Modified: 20 Jun 2015 04:59
URI: http://eprints.goums.ac.ir/id/eprint/1785

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