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Discrimination of Entamoeba spp. in children with dysentery

Sharbatkhori, M. and Nazemalhosseini-Mojarad, E. and Cheraghali, F. and Maghsoodloorad, F.S. and Taherkhani, H. and Vakili, M. (2014) Discrimination of Entamoeba spp. in children with dysentery. Gastroenterology and Hepatology from Bed to Bench, 7 (3). pp. 164-167. ISSN 20082258 (ISSN)

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Abstract

Aim: The present study was performed in order to differentiate E. histolytica and E. dispar in children from Gorgan city, using a PCR method. Background: Differential detection of two morphologically indistinguishable protozoan parasites Entamoeba histolytica and E. dispar has a great clinical and epidemiological importance because of potential invasive pathogenic E. histolytica and non-invasive parasite E. dispar. Patients and methods: One hundred and five dysentery samples were collected from children hospitalized in Taleghani hospital in Gorgan city. The fecal specimens were examined by light microscopy (10X then 40X) to distinguish Entamoeba complex. A single round PCR amplifying partial small-subunit rRNA gene was performed on positive microscopy samples to differentiate E. histolytica/ E. dispar and E. moshkovskii from each other. Results: Twenty-five specimens (23.8%) were positive for Enramoeba complex in direct microscopic examination. PCR using positive controls indicated E. histolytica and E. dispar in two (2/25, 8%) and three (3/25, 12%) samples, respectively. Conclusion: There is a warrant to performing molecular diagnosis for stool examination at least in hospitalized children in order to prevent incorrect reports from laboratories and consequently mistreating by physicians. © 2014 RIGLD, Research Institute for Gastroenterology and Liver Diseases.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Gastroenterol. Hepatol. Bed Bench [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, School of Medicine, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Gastroenterology and Liver Disease Research center, Shahid Beheshti University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Department of Pediatric Diseases, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Community Medicine, School of Medicine, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Children, Dysentery, Entamoeba dispar, Entamoeba histolytica, Gorgan, Iran, PCR, amebiasis, Article, child, controlled study, dysentery, Entamoeba, Entamoeba dispar, Entamoeba histolytica, Entamoeba moshkovskii, feces analysis, human, Iran, major clinical study, microscopy, molecular diagnosis, nonhuman, polymerase chain reaction, species differentiation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:26
Last Modified: 09 May 2015 06:23
URI: http://eprints.goums.ac.ir/id/eprint/1784

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