Golestan University of Medical Sciences Repository

Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran

Moradi, A. and Zhand, S. and Ghaemi, A. and Javid, N. and Bazouri, M. and Tabarraei, A. (2014) Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran. Iranian Journal of Basic Medical Sciences, 17 (5). pp. 370-377. ISSN 20083866 (ISSN)

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Abstract

Objective(s): It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients. Materials and Methods: In this study, 120 chronic HBV patients from Golestan, Northeast of Iran who were not vaccinated against HBV, were recruited from the year 2008 to 2012. HBV-DNA extraction from plasma and PCR were performed and positive PCR products were subjected to automated sequencing. Results: One hundred out of 120 (83.3%) patients were HBeAg negative. Comparison of our nucleotide sequences with reference sequence showed high rate mutation in BCP and PC region (96.66%). Frame shift mutation was found in 78 (65%) of patients in BCP region, among them 8 (6.6%) patients showed mutation in PC region. Conclusion: Our results demonstrated high rate of mutations in BCP and PC regions among HBV chronic patients in Northeast of Iran.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Basic Med. Sci. [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: BCP mutation, Hepatitis B, Iran, PC mutation, hepatitis B core antigen, protein, unclassified drug, X gene protein, adult, article, basal core promoter region, DNA extraction, DNA sequence, enzyme linked immunosorbent assay, female, frameshift mutation, gene amplification, hepatitis B, Hepatitis B virus, human, Iran, major clinical study, male, missense mutation, mutation, nucleotide sequence, point mutation, polymerase chain reaction, pre core promoter region, promoter region, stop codon
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:46
Last Modified: 06 May 2015 07:37
URI: http://eprints.goums.ac.ir/id/eprint/1776

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