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Cryptosporidium infection in patients with gastroenteritis in Sari, Iran

Gholami, S. and Khanmohammadi, M. and Ahmadpour, E. and Paqhe, E.S. and Nakhjiri, S.K. and Ramazannipour, H. and Shahbazi, A. (2014) Cryptosporidium infection in patients with gastroenteritis in Sari, Iran. Iranian Journal of Parasitology, 9 (2). pp. 226-232. ISSN 17357020 (ISSN)

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Abstract

Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore, the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran. Methods: Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacidfast staining (AFS) and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium. Results: In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp. Conclusion: The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Parasitol. [Field not mapped to EPrints] AD - Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Iran [Field not mapped to EPrints] AD - Golestan University of Medical Sciences, Iran [Field not mapped to EPrints] AD - University of Mazandaran, Iran [Field not mapped to EPrints] AD - Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical sciences, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cryptosporidium spp., Gastroenteritis Patients, Nested PCR, genomic DNA, ribosome RNA, article, clinical feature, cross-sectional study, cryptosporidiosis, Cryptosporidium, dehydration, feces analysis, female, fever, gastroenteritis, genetic analysis, human, Iran, major clinical study, male, nausea, oocyst, polymerase chain reaction, restriction fragment length polymorphism, vomiting, weight reduction
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 06:43
Last Modified: 04 May 2015 09:50
URI: http://eprints.goums.ac.ir/id/eprint/1772

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