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Antihaemolytic activity of thirty herbal extracts in mouse red blood cells

Khalili, M. and Ebrahimzadeh, M.A. and Safdari, Y. (2014) Antihaemolytic activity of thirty herbal extracts in mouse red blood cells. Arhiv za Higijenu Rada i Toksikologiju, 65 (4). pp. 399-406. ISSN 00041254 (ISSN)

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Abstract

Reactive oxygen species (ROS) can lead to haemolysis and eventually to diseases such as thalassemia and sickle cell anaemia. Their action can be counteracted by the antihaemolytic activity of therapeutic agents. The aim of our study was to identify plants that most efficiently counteract ROS-caused haemolysis. From ten plants known for their antioxidant activity (Orobanche orientalis G. Beck, Cucumis melo L., Albizzia julibrissin Durazz, Galium verum L., Scutellaria tournefortii Benth, Crocus caspius Fischer & Meyer, Sambucus ebulus L., Danae racemosa L., Rubus fruticsos L., and Artemisia absinthium L.) we prepared 30 extracts using three extraction methods (percolation, Soxhlet, and ultrasound-Assisted extraction) to see whether the extraction method affects antihaemolytic efficiency, and one extraction method (polyphenol extraction) to see how much of this action is phenol-related. Extract antihaemolytic activity was determined in mice red blood cells and compared to that of vitamin C as a known antioxidant. Nine of our extracts were more potent than vitamin C, of which G. verum (aerial parts/percolation) and S. tournefortii (aerial parts/polyphenol) extracts were the most potent, with an IC50 of 1.32 and 2.08 μg mL-1, respectively. Haemolysis inhibition depended on extract concentration and the method of extraction. These plants could provide accessible sources of natural antioxidants to the pharmaceutical industry © 2015 Institute.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Arh. Hig. Rada Toksikol. [Field not mapped to EPrints] AD - Mazandaran University of Medical Sciences, School of PharmacySari, Iran [Field not mapped to EPrints] AD - Faculty of Advanced Medical Technologies, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: fenoli, Galium verum, perkolacija, Scutellaria tournefortii, Soxhlet, ultrazvu�na ekstrakcija, vodikov peroksid, albizzia julibrissin extract, antioxidant, Artemisia absinthium extract, ascorbic acid, crocus caspius extract, cucumis melo extract, danae racemosa extract, galium verum extract, hemolytic agent, orobanche orientalis extract, plant extract, rubus fruticsos extract, sambucus ebulus extract, scutellaria tournefortii extract, unclassified drug, animal experiment, antioxidant activity, Article, controlled study, erythrocyte, hemolysis, IC50, male, mouse, nonhuman, Soxhlet extraction, soybean, Albizia julibrissin, Artemisia absinthium, Crocus, Cucumis melo, Danae racemosa, Galium verum, Mus, Orobanche, Rubus, Sambucus ebulus, Scutellaria
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:09
Last Modified: 05 May 2015 04:34
URI: http://eprints.goums.ac.ir/id/eprint/1771

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