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Effect of gestational diabetes on neuronal cells in rat cerebellum in early postnatal life

Razi, E.M. and Ghafari, S. and Hojati, V. and Golalipour, M.J. (2014) Effect of gestational diabetes on neuronal cells in rat cerebellum in early postnatal life. International Journal of Morphology, 32 (2). pp. 420-425. ISSN 07179367 (ISSN)

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Abstract

Previous study has shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal model. This study was conducted to determine the effect of gestational diabetes on rat cerebellum in early postnatal life. In this experimental study, 10 dams randomly allocated into control and diabetic groups on day 1 of gestation. Five dams in diabetic group were administered 40 mg/kg/BW (intraperitoneally) of streptozotocin and control animals received normal saline. Six offspring of each gestational diabetes mellitus and controls were randomly selected at day 7 of postnatal life. Offspring were sacrificed and coronal sections were taken from the cerebellum and stained with cresyl violet. The number of Purkinje and granular cells and thickness of layers of cerebellum were evaluated by quantitative computer-assisted morphometric method. The Purkinje cells density at apex and depth of cerebellar lobules in the experimental group (14.40±0.7, 14.86±0.6) significantly reduced in comparison with the control group (16.72±0.3, 17.85±0.7) (P<0.05). The granular cell density at apex and depth of cerebellar lobules in the experimental group (23.94±0.6, 22.81±0.5) significantly reduced in comparison with the control group (29.20±0.8, 28.1±0.8) (P<0.05). The thickness of the Purkinje and internal granular and molecular layers at apex and depth of cerebellar cortex significantly reduced in diabetics group compared to controls (P<0.05). This study revealed that gestational diabetes induces loss of number and size of the Purkinje cells and the granular cells and reduction of thickness of the Purkinje and internal granular layer of the cerebellar cortex in neonatal mice.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English; Spanish [Field not mapped to EPrints] ST - Efecto de la diabetes gestacional sobre las células neuronales del cerebelo de ratas en el período postnatal [Field not mapped to EPrints] J2 - Int. J. Morphol. [Field not mapped to EPrints] AD - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran [Field not mapped to EPrints] AD - Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Cerebellum, Purkinje cell, Gestational diabetes, Granular cell, Rat
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:53
Last Modified: 09 May 2015 05:15
URI: http://eprints.goums.ac.ir/id/eprint/1765

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