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Control of expression of the RNases J1 and J2 in bacillus subtilis

Jamalli, A. and Hébert, A. and Zig, L. and Putzer, H. (2014) Control of expression of the RNases J1 and J2 in bacillus subtilis. Journal of Bacteriology, 196 (2). pp. 318-324. ISSN 00219193 (ISSN)

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In Bacillus subtilis, the dual activity 5' exo- and endoribonucleases J1 and J2 are important players in mRNA and stable RNA maturation and degradation. Recent work has improved our understanding of their structure and mechanism of action and identified numerous RNA substrates. However, almost nothing is known about the expression of these enzymes. Here, we have identified the transcriptional and translational signals that control the expression of the rnjA (RNase J1) and rnjB (RNase J2) genes. While the rnjB gene is transcribed constitutively from a sigma A promoter, optimal expression of RNase J1 requires cotranscription and cotranslation with the upstream ykzG gene, encoding a protein of unknown function. In the absence of coupled translation, RNase J1 expression is decreased more than 5-fold. Transcription of the ykzG operon initiates at a sigma A promoter with a noncanonical -35 box that is required for optimal transcription. Biosynthesis of RNase J1 is autocontrolled within a small range (1.4-fold) and also slightly stimulated (1.4-fold) in the absence of RNase J2. These controls are weak but might be useful to maintain the overall RNase J level and possibly also equimolar amounts of the two nucleases in the cell that primarily act as a heterodimer in vivo. © 2014, American Society for Microbiology.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Bacteriol. [Field not mapped to EPrints] AD - CNRS UPR 9073, Institut de Biologie Physico-Chimique, Paris, France [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - IFP Energies Nouvelles, Biotechnology Department, Rueil-Malmaison, France [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: heterodimer, ribonuclease, ribonuclease j1, ribonuclease j2, unclassified drug, article, Bacillus subtilis, consensus sequence, controlled study, enzyme regulation, enzyme synthesis, in vivo study, nonhuman, point mutation, priority journal, promoter region, protein expression, start codon, stop codon, transcription initiation, translation initiation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 07:41
Last Modified: 26 Feb 2018 05:26
URI: http://eprints.goums.ac.ir/id/eprint/1762

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