Bazmani, A. and Khanmohammadi, M. and Nakhjiri, S.K. and Shahbazi, M.R. and Ramazanipoor, H.R. and Bafandeh, M. and Rasouli, A. and Shahbazi, A. (2014) Diversity of Merozoite Surface Protein-3α Gene of plasmodium vivax in isolates from Sistan and Bluchestan provinces in Iran. Journal of Pure and Applied Microbiology, 8 (2). pp. 1133-1136. ISSN 09737510 (ISSN)
Full text not available from this repository.Abstract
Approximately 90 million people are suffered from vivax malaria annually. This disease imposes a heavy economic cost in endemic areas of Asia and the Americas. This study was design and implemented to reveal the extent of polymorphism of the Plasmodium vivax Merozoite Surface Protein -3αgene. 37 blood samples (20 Iranian and 17 non-Iranian) collected from patients attending to malaria clinics in the Sistan and Baluchistan province. The DNA was amplified by nested PCR and the products were digested with HhaI enzyme through PCR/RFLP technique. Three biotypes of the gene based on the size of PCR products, including A (about 1900bp), B (about 1400bp) and C (about 1100bp) and 10 allelic groups after digesting of PCR products with HhaI enzyme was observed. A significant difference between the two groups of patients was not detected.We observed that P. vivax isolates of Sistan and Bluchestan were extremely diverse, and the results are almost compatible with the results of other studies performed previously. Based on the results of our study RFLP method using HhaI (small fragments from 50- 500bp) is suitable technique for describing diversity of the gene.
| Item Type: | Article |
|---|---|
| Additional Information: | Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Pure Appl. Microbiol. [Field not mapped to EPrints] AD - Tabriz Research Centre of Infectious and Tropical Diseases, Tabriz University of Medical SciencesTabriz, Iran [Field not mapped to EPrints] AD - Department of Laboratory Sciences, Marand Branch, Islamic Azad UniversityMarand, Iran [Field not mapped to EPrints] AD - Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Iran [Field not mapped to EPrints] AD - Department of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad UniversityKaraj, Iran [Field not mapped to EPrints] AD - Faculty of Basic Sciences, University of MazandaranSari, Iran [Field not mapped to EPrints] AD - Immunology Research Centre, Tabriz University of Medical SciencesTabriz, Iran [Field not mapped to EPrints] AD - Drug Applied Research Center, Tabriz University of Medical SciencesTabriz, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] |
| Uncontrolled Keywords: | Diversity, Iran, Plasmodium vivax, Sistan and bluchestan, merozoite surface protein 3alpha, microorganism protein, parasite antigen, protozoal protein, unclassified drug, allele, Article, biotype, clinical article, gene frequency, genetic polymorphism, genetic variability, genotype, human, human tissue, merozoite, microbial diversity, nonhuman, Plasmodium vivax, polymerase chain reaction, protozoal genetics, restriction fragment length polymorphism, vaccine production |
| Subjects: | مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus |
| Divisions: | معاونت تحقیقات و فناوری |
| Depositing User: | GOUMS |
| Date Deposited: | 19 Apr 2015 05:03 |
| Last Modified: | 20 Jun 2015 06:40 |
| URI: | http://eprints.goums.ac.ir/id/eprint/1759 |
Actions (login required)
 |
View Item |
