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Study of the antibacterial activity of total extract and Petroleum ether, chloroform, ethyl acetate and aqueous fractions of aerial parts of heliotropium bacciferum against staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, E.coli, Salmonella enteritidis

Rahimifard, N. and Bagheri, E. and Asgarpanah, J. and Balajadeh, B.K. and Yazdi, H. and Bagheri, F. (2014) Study of the antibacterial activity of total extract and Petroleum ether, chloroform, ethyl acetate and aqueous fractions of aerial parts of heliotropium bacciferum against staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, E.coli, Salmonella enteritidis. Biosciences Biotechnology Research Asia, 11 (1). pp. 239-248. ISSN 09731245 (ISSN)

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Abstract

Heliotropium bacciferum is One of the plants belonging to the family Boraginaceae , which is Restricted distribution in the south of Iran. It is used for Hypotension, fever, stomach ulcers in traditional medicine. In this study, the antibacterial effects of extracts and fractions of chloroform, ethyl acetate and aqueous, aerial parts of Heliotropium bacciferum Forssk was evaluated against five bacterial strains. The methanol extract were prepared using the percolation method. Fractions of chloroform, Petroleum ether, ethyl acetate, methanol and aqueous respectively by Liquid - Liquid fractionation of the total extract were prepared. The antibacterial activity against two Gram positive bacteria, three Gram negative bacterial using Minimum inhibitory concentration in microplate and well plate method. Results showed that H. bacciferum extracts exhibited a significant activity against strains Staphylococcus aureus, Bacillus cereus,Pseudomonas aeruginosa, E.coli, Salmonella enteritidis. MIC and well plate is between 7.6-125 μg/ml. The results of this study indicate that extracts of the plant H.bacciferum has a antimicrobial effect against strains are listed And among the extracts, aqueous part is that most antibacterial effect of the other fraction and then methanolic extract has the greatest effect.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Biosci. Biotechnol. Res. Asia [Field not mapped to EPrints] AD - Food and Drug Laboratory Research Center (FDLRC), Islamic Azad University, Ministry of Health (MOH), Tehran, Iran [Field not mapped to EPrints] AD - Department of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran [Field not mapped to EPrints] AD - Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran [Field not mapped to EPrints] AD - Department of Pharmacology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Antimicrobial activity, Heliotropium bacciferum, MIC, Well plate, acetic acid ethyl ester, antibiotic agent, cefalexin, chloroform, gentamicin, Heliotropium bacciferum extract, petroleum ether, plant extract, unclassified drug, antibacterial activity, article, Bacillus cereus, bacterial strain, controlled study, Escherichia coli, fractionation, Heliotropium, Heliotropium bacciferum, minimum inhibitory concentration, nonhuman, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 10:04
Last Modified: 09 May 2015 04:51
URI: http://eprints.goums.ac.ir/id/eprint/1757

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