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Growth-inhibitory and apoptosis-inducing effects of Punica Granatum L. var. spinosa (apple punice) on fibrosarcoma cell lines

Sepehr, K.S. and Baradaran, B. and Mazandarani, M. and Yousefi, B. and Alitappeh, M.A. and Khori, V. (2014) Growth-inhibitory and apoptosis-inducing effects of Punica Granatum L. var. spinosa (apple punice) on fibrosarcoma cell lines. Advanced Pharmaceutical Bulletin, 4. pp. 583-590. ISSN 22285881 (ISSN)

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Purpose: Punica granatum L. var. granatum (Pomegranate), an herbaceous plant found in Iran, The aim of this study was to investigate the cytotoxic effects, induction of apoptosis, and the mechanism of cell death of ethanol extract from Punica granatum L. var. spinosa on the mouse fibrosarcoma cell line, WEHI-164. Methods: Various parts of the herbs were extracted from fruit using ethanol as the solvent, and the cytotoxicity and cell viability of the ethanolic extract were determined by the MTT assay. To determine whether necrosis or apoptosis is the predominant cause of cell death, cell death detection was performed using the ELISA method. The induction of apoptosis was confirmed using the terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) assay. Moreover, a sensitive immunoblotting technique was used to examine the production of Caspase-3 and Bcl2 proteins. Results: Our findings suggested that the ethalonic extract of Punica granatum L. var. spinosa altered cell morphology, decreased cell viability, suppressed cell proliferation and induced cell death in a time- and dose-dependent manner in WEHI-164 cells (IC50 = 229.024μg/ml), when compared to a chemotherapeutic anticancer drug, Toxol (Vesper Pharmaceuticals), with increased nucleosome production from apoptotic cells. Induction of apoptosis by the plant extract was proved by the decrease of pro-Caspase-3 and Bcl2 proteins and quantitatively confirmed by Immunoblotting analysis. Conclusion: The results obtained from the present study have demonstrated the growth-inhibitory effect of Ethanol Extracts from Punica granatum L. var. spinosa, and clearly showed that apoptosis was the major mechanism of in-vitro cell death induced by the extract. © 2014 The Authors.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Adv. Pharm. Bull. [Field not mapped to EPrints] AD - Immunology Research Center (IRC), Tabriz University of Medical SciencesTabriz, Iran [Field not mapped to EPrints] AD - Department of Botany, Gorgan Branch, Islamic Azad UniversityGorgan, Iran [Field not mapped to EPrints] AD - Ischemic Disorders Research Center, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Apoptosis, Fibrosarcoma cell line, Punica granatum L. var. spinosa, 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, caspase 3, paclitaxel, pomegranate extract, protein bcl 2, animal cell, antineoplastic activity, apoptosis, Article, cancer cell destruction, cancer inhibition, cell proliferation, cell structure, cell viability, controlled study, drug cytotoxicity, drug mechanism, drug research, enzyme linked immunosorbent assay, fibrosarcoma cell line, fruit, immunoblotting, mouse, nick end labeling, nonhuman
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 08:48
Last Modified: 26 Feb 2018 05:16
URI: http://eprints.goums.ac.ir/id/eprint/1749

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