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Alleviation of experimental allergic encephalomyelitis in C57BL/6 mice by Soy Daidzein

Jahromi, S.R. and Arrefhosseini, S.R. and Ghaemi, A. and Alizadeh, A. and Tabriz, H.M. and Togha, M. (2014) Alleviation of experimental allergic encephalomyelitis in C57BL/6 mice by Soy Daidzein. Iranian Journal of Allergy, Asthma and Immunology, 13 (4). pp. 256-264. ISSN 17351502 (ISSN)

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Abstract

Experimental allergic encephalomyelitis (EAE) is considered as the murine model of multiple sclerosis. Daidzein a phytostrogenic compound of soy is known to impose immunomodulatory and antioxidative effects. We conducted this study to assess the potential protective and therapeutic effects of daidzein on allergic encephalomyelitis. C57BL/6 mice were induced with allergic encephalomyelitis using myelin oligodendrocyte glycoprotein (35-55) and received daidzein or dimethyl sulfoxide as the vehicle control. To assess the protective effect of daidzein, the mice were administered with 20 mg/kg of daidzein from 21 days prior to 21 days post EAE induction on a daily basis. To evaluate the therapeutic effect of daidzein, mice were fed with 300 mg/kg daidzein after the appearance of the first clinical signs for 10 days. One day after the last gavage, the mice were sacrificed. Spleen and brain were removed for further histological and immunological analysis. Feeding mice with low dose of daidzein prior to disease induction did not affect disease severity. However, treating with high dose of daidzein after the onset of the disease reduced interferon-3 and interleukin-12 secretion, enhanced interleukin-10 production, suppressed lymphocyte proliferation, and decreased cytotoxicity as judged by lactate dehydrogenase release. In conclusion, daidzein reduced the extent of demyelination and disease severity. Chronic oral therapy with low dose of daidzein did not prevent experimental autoimmune encephalomyelitis. However, high doses of daidzein could prohibit disease exacerbation. Copyright© Summer 2014, Iran J Allergy Asthma Immunol. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. J. Allergy Asthma Immunol. [Field not mapped to EPrints] C2 - 24659161 [Field not mapped to EPrints] AD - Sina Hospital, Multiple Sclerosis Research Center-Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research Center, Tehran, Iran [Field not mapped to EPrints] AD - Department of Nutrition and Health, Tabriz University of Medical Sciences, Tabriz, Iran [Field not mapped to EPrints] AD - Infectious Diseases Research Center, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Tissue Engineering Department, Advanced Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Pathology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Department of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] AD - Iranian Center of Neurological Research-Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Daidzein, Experimental allergic encephalomyelitis (EAE), Immunomodulation, Interferon-gamma, Isoflavones, Multiple sclerosis, Soy, daidzein, dimethyl sulfoxide, gamma interferon, interleukin 10, interleukin 12, lactate dehydrogenase, myelin oligodendrocyte glycoprotein, cytokine, daidzein, isoflavone derivative, allergic encephalomyelitis, animal cell, animal experiment, animal model, animal tissue, article, cell count, cell suspension, controlled study, cytokine production, cytokine release, cytotoxicity, demyelination, disease severity, drug bioavailability, drug megadose, experimental autoimmune encephalomyelitis, female, histopathology, lymphocyte proliferation, mononuclear cell, mouse, nonhuman, spleen cell, animal, C57BL mouse, Encephalomyelitis, Autoimmune, Experimental, immunology, lymphocyte activation, secretion (process), T lymphocyte, Animals, Cytokines, Encephalomyelitis, Autoimmune, Experimental, Female, Isoflavones, Lymphocyte Activation, Mice, Mice, Inbred C57BL, T-Lymphocytes
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:41
Last Modified: 04 May 2015 16:10
URI: http://eprints.goums.ac.ir/id/eprint/1747

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