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Molecular variation in Leishmania parasites from sandflies species of a zoonotic cutaneous leishmaniasis in northeast of Iran

Sharbatkhori, M. and Spotin, A. and Taherkhani, H. and Roshanghalb, M. and Parvizi, P. (2014) Molecular variation in Leishmania parasites from sandflies species of a zoonotic cutaneous leishmaniasis in northeast of Iran. Journal of Vector Borne Diseases, 51 (1). pp. 16-21. ISSN 09729062 (ISSN)

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Abstract

Background & objectives: In the well-known zoonotic cutaneous leishmaniasis (ZCL) focus in Turkmen Sahara, border of Iran and Turkmenistan, ZCL has increased among humans in the past five years. The present study was undertaken to incriminate vectors of ZCL in the region, and to find molecular variation in Leishmania parasites. Methods: The sandflies were sampled using CDC light-traps and sticky papers. All the sandflies were identified using morphological characters of the head and abdominal terminalia. DNA was extracted from the dissected thorax and attached anterior abdomen of individual female sandfly. Leishmania detection and identification of sandflies were performed using PCR, digestion of BsuRI restriction enzyme and sequencing of ITS-rDNA gene and also by semi-nested PCR to amplify minicircle kinetoplast (k) DNA of Leishmania. Results: Leishmania infections were detected in 26 out of 206 female sandflies. Of the infected sandflies, 18 were Phlebotomus papatasi while eight were P. caucasicus/P. mongolensis. Two infections of L. turnica were detected, one in P. papatasi and other in P. caucasicus/P. mongolensis and the rest of the sandflies were found infected with L. major. Conclusion: Our finding showed that L. major had low diversity with only one common haplotype (GenBank Access No. EF413075). The novel haplotypes were discovered in L. major (GenBank Access No. KF152937) and in L. turanica (GenBank Access No. EF413079) in low frequency. These Leishmania parasites are circulating to maintain infections in the P. papatasi and P. caucasicus/P. mongolensis in Turkmen Sahara.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Vector Borne Dis. [Field not mapped to EPrints] AD - Laboratory Science Research Centre, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Parasitology and Mycology, Schoolof Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Molecular Systematics Laboratory, Parasitology Department, Pasteur Instituteof Iran, Tehran, Iran [Field not mapped to EPrints] AD - Parasitology Department, Tabriz University of Medical Sciences, Tabriz, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Iran, ITS ribosomal DNA, L. turanica, Leishmania major, P. caucasicus/P. mongolensis, Phlebotomus papatasi, internal transcribed spacer, article, controlled study, DNA extraction, female, Iran, Leishmania, Leishmania major, Leishmania turnica, nonhuman, nucleotide sequence, parasite identification, parasite vector, Phlebotomus, Phlebotomus caucasicus, Phlebotomus mongolensis, Phlebotomus papatasi, polymerase chain reaction, restriction fragment length polymorphism, skin leishmaniasis
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 19 Apr 2015 05:15
Last Modified: 09 May 2015 06:25
URI: http://eprints.goums.ac.ir/id/eprint/1741

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