Golestan University of Medical Sciences Repository

Two novel splice variants of SOX2OT, SOX2OT-S1, and SOX2OT-S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma

Shahryari, A. and Rafiee, M.R. and Fouani, Y. and Oliae, N.A. and Samaei, N.M. and Shafiee, M. and Semnani, S. and Vasei, M. and Mowla, S.J. (2014) Two novel splice variants of SOX2OT, SOX2OT-S1, and SOX2OT-S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma. Stem Cells, 32 (1). pp. 126-134. ISSN 10665099 (ISSN)

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Abstract

Long noncoding RNAs (lncRNAs) have emerged as new regulators of stem cell pluripotency and tumorigenesis. The SOX2 gene, a master regulator of pluripotency, is embedded within the third intron of a lncRNA known as SOX2 overlapping transcript (SOX2OT). SOX2OT has been suspected to participate in regulation of SOX2 expression and/or other related processes; nevertheless, its potential involvement in tumor initiation and/or progression is unclear. Here, we have evaluated a possible correlation between expression patterns of SOX2OT and those of master regulators of pluripotency, SOX2 and OCT4, in esophageal squamous cell carcinoma (ESCC) tissue samples. We have also examined its potential function in the human embryonic carcinoma stem cell line, NTERA2 (NT2), which highly expresses SOX2OT, SOX2, and OCT4. Our data revealed a significant coupregulation of SOX2OT along with SOX2 and OCT4 in tumor samples, compared to the non-tumor tissues obtained from the margin of same tumors. We also identified two novel splice variants of SOX2OT (SOX2OT-S1 and SOX2OT-S2) which coupregulated with SOX2 and OCT4 in ESCCs. Suppressing SOX2OT variants caused a profound alteration in cell cycle distribution, including a 5.9 and 6.9 time increase in sub-G1 phase of cell cycle for SOX2OT-S1 and SOX2OT-S2, respectively. The expression of all variants was significantly diminished, upon the induction of neural differentiation in NT2 cells, suggesting their potential functional links to the undifferentiated state of the cells. Our data suggest a part for SOX2OT spliced variants in tumor initiation and/or progression as well as regulating pluripotent state of stem cells.© AlphaMed Press 2013.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Stem Cells [Field not mapped to EPrints] C2 - 24105929 [Field not mapped to EPrints] AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran [Field not mapped to EPrints] AD - Department of Medical Genetics, Faculty of Advanced Medical Technologies, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Pathology, Shariati Hospital, University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: ESCC, LncRNA, Novel spliced variants, SOX2, SOX2OT, Stem cell, octamer transcription factor 4, sox2 overlapping transcript s1, sox2 overlapping transcript s2, transcription factor Sox2, unclassified drug, article, cancer growth, cell cycle, cell cycle G1 phase, cell differentiation, cellular distribution, controlled study, down regulation, embryonal carcinoma stem cell, esophageal squamous cell carcinoma, exon, human, human cell, nucleotide sequence, pluripotent stem cell, protein expression, protein function, protein stability, upregulation, ESCC, LncRNA, Novel spliced variants, SOX2, SOX2OT, Stem cell, Carcinoma, Squamous Cell, Cell Culture Techniques, Cell Differentiation, Embryonal Carcinoma Stem Cells, Esophageal Neoplasms, Humans, Octamer Transcription Factor-3, Pluripotent Stem Cells, Protein Isoforms, RNA Interference, RNA Splicing, RNA, Long Noncoding, SOXB1 Transcription Factors, Up-Regulation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 04:47
Last Modified: 09 May 2015 06:02
URI: http://eprints.goums.ac.ir/id/eprint/1737

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