Golestan University of Medical Sciences Repository

The effect of MDMA-induced anxiety on neuronal apoptosis in adult male rats' hippocampus

Karimi, S. and Jahanshahi, M. and Golalipour, M.J. (2014) The effect of MDMA-induced anxiety on neuronal apoptosis in adult male rats' hippocampus. Folia Biologica (Czech Republic), 60 (4). pp. 187-191. ISSN 00155500 (ISSN)

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Abstract

Ecstasy or MDMA as a psychoactive drug and hallucinogen is considered one of the most commonly used drugs in the world. This psychotropic substance is discussed both as sexually stimulating and reducing fear and anxiety. Amphetamines also destroy neurons in some brain areas. The aim of this study was to investigate the effects of MDMA on anxiety and apoptosis of hippocampal neurons. Fortytwo male Wistar rats of mean weight 200-220 g were used and distributed into six groups [control, con-trol-saline, and experimental groups (1.25, 2.5, 5, 10 mg/kg)]. Rats in experimental groups received MDMA at different doses for seven days by intraperitoneal injection and the control-saline group received saline (1 ml/kg); anxiety was then investigated by plus-maze test. Forty-eight hours after behavioural testing brains were taken from animals and fixed, and after tissue processing, slices were stained with TUNEL kit for apoptotic cells. The area densities of apoptotic neurons were measured throughout the hippocampus and compared in all groups (P < 0.05). Physiological studies showed that 1.25 mg/kg and 2.5 mg/kg doses caused anti-Anxiety behaviour and 5 and 10 mg/kg doses of MDMA caused anxietylike behaviour. Moreover, our histological study showed that ecstasy increased apoptotic cell numbers and the highest increase was observed with the 10 mg/kg dose of MDMA. We concluded that MDMA can cause different responses of anxiety-like behaviour in different doses. This phenomenon causes a different ratio of apoptosis in hippocampal formation. Reduction of anxiety-like behaviour induced by the 2.5 mg/kg dose of MDMA can control apoptosis.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Folia Biol. [Field not mapped to EPrints] AD - Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, km 4 Gorgan-Sari road(Shastcola), Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: 3,4 methylenedioxymethamphetamine, animal cell, animal experiment, animal model, anxiety disorder, apoptosis, Article, behavior, brain histology, brain nerve cell, cell count, cell density, controlled study, dose response, hippocampus, male, maze test, nick end labeling, nonhuman, rat
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 04:16
Last Modified: 20 Jun 2015 05:13
URI: http://eprints.goums.ac.ir/id/eprint/1732

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