Golestan University of Medical Sciences Repository

PCR-based identification of methicillin-resistant Staphylococcus aureus strains and their antibiotic resistance profiles

Pournajaf, A. and Ardebili, A. and Goudarzi, L. and Khodabandeh, M. and Narimani, T. and Abbaszadeh, H. (2014) PCR-based identification of methicillin-resistant Staphylococcus aureus strains and their antibiotic resistance profiles. Asian Pacific Journal of Tropical Biomedicine, 4. S293-S297. ISSN 22211691 (ISSN)

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Abstract

Objective: To evaluated the PCR for mecA gene compared with the conventional oxacillin disk diffusion method for methicillin-resistant Staphylococcus aureus (S. aureus) identification. Methods: A total of 292 S. aureus strains were isolated from various clinical specimens obtained from hospitalized patients. Susceptibility test to several antimicrobial agents was performed by disk diffusion agar according to Clinical and Laboratory Standards Institute guidelines. The PCR amplification of the mecA gene was carried out in all the clinical isolates. Results: Among antibiotics used in our study, penicillin showed the least anti-staphylococcal activity and vancomycin was the most effective. The rate of methicillin-resistant S. aureus prevalence determined by oxacillin disk diffusion method was 47.6%; whereas, 45.1% of S. aureus isolates were mecA- positive in the PCR assay. Conclusions: This study is suggestive that the PCR for detection of mecA gene is a fast, accurate and valuable diagnostic tool, particularly in hospitals in areas where methicillin-resistant S. aureus is endemic. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Asian Pac. J. Trop. Biomed. [Field not mapped to EPrints] AD - Department of Microbiology, Faculty of Medicine, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Faculty of Medicine, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical ScienceTehran, Iran [Field not mapped to EPrints] AD - Department of Pediatric Infectious Disease, Faculty of Medicine, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Department of Microbiology, Faculty of Medicine, Iran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Department of Pharmacology, Faculty of Medicine, Iran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: MecA gene, Methicillin-resistant staphylococcus aureus, Oxacillin disk diffusion, PCR, oxacillin, penicillin binding protein 2a, penicillin derivative, vancomycin, antibiotic resistance, antibiotic sensitivity, Article, bacterial strain, bacterium identification, bacterium isolation, controlled study, disk diffusion, intermethod comparison, mecA gene, methicillin resistant Staphylococcus aureus, nonhuman, polymerase chain reaction, Staphylococcus aureus, methicillin resistant Staphylococcus aureus, Staphylococcus aureus
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 09:20
Last Modified: 23 May 2015 08:30
URI: http://eprints.goums.ac.ir/id/eprint/1720

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