Golestan University of Medical Sciences Repository

Nanomolecular detection of human influenza virus type A using reverse transcription loop-mediated isothermal amplification assisted with rod-shaped gold nanoparticles

Nikbakht, H. and Gill, P. and Tabarraei, A. and Niazi, A. (2014) Nanomolecular detection of human influenza virus type A using reverse transcription loop-mediated isothermal amplification assisted with rod-shaped gold nanoparticles. RSC Advances, 4 (26). pp. 13575-13580. ISSN 20462069 (ISSN)

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Abstract

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) and rod-shaped gold nanoparticles (gold nanorods; GNRs) were employed for nanomolecular detection of human influenza virus type A RNA. After cDNA synthesis from the RNA, the primers targeting the M protein gene were used for LAMP amplification. A blue shift from red to purple from the GNR inserting into the LAMP-DNAs can be seen by the naked eye. Transmission electron microscopy revealed the formation GNR aggregates due to their interactions with LAMP DNA. One pg RNA (10-3 dilution of the viral cDNA) was detected using this colorimetric test. The nanomolecular test showed 100% sensitivity and 95.8% specificity in comparison to results by RT-PCR. Also, the test indicated 100% sensitivity and 100% specificity in comparison to results by RT-LAMP. The described nanomolecular test could detect human influenza virus type A RNA in nearly 1 hour. This journal is © the Partner Organisations 2014.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - RSC Adv. [Field not mapped to EPrints] AD - Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran [Field not mapped to EPrints] AD - Department of Virology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Biosynthesis, Gold, Isotherms, Metal nanoparticles, Nanorods, Polymerase chain reaction, RNA, Transcription, Transmission electron microscopy, Viruses, cDNA synthesis, Gold Nanoparticles, Gold nanorod, Human influenza virus, Loop-mediated isothermal amplifications, Protein genes, Reverse transcription, Rod-shaped, Nanoribbons
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:11
Last Modified: 14 May 2015 16:04
URI: http://eprints.goums.ac.ir/id/eprint/1719

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