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Immunogenicity evaluation of a DNA vaccine expressing the hepatitis C virus non-structural protein 2 gene in C57BL/6 Mice

Gorzin, Z. and Gorzin, A.A. and Tabarraei, A. and Behnampour, N. and Irani, S. and Ghaemi, A. (2014) Immunogenicity evaluation of a DNA vaccine expressing the hepatitis C virus non-structural protein 2 gene in C57BL/6 Mice. Iranian Biomedical Journal, 18 (1). pp. 1-7. ISSN 1028852X (ISSN)

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Abstract

Backgrounds: Most of the hepatitis C virus (HCV) infections elicit poor immune responses and 75% to 85% of cases become chronic; therefore, the development of an effective vaccine against HCV is of paramount importance. In this study, we aimed to evaluate co-administration of HCV non-Structural Protein 2 and IL-12 DNA vaccines in C57BL/6 mice. Methods: A plasmid encoding full-length HCV NS2 protein (non-structural protein 2) was generated and used to vaccinate mice. Negative control (an empty expression vector) was also employed to evaluate the background response. To investigate immune responses against vaccine, C57BL/6 mice received three doses of the vaccine with a two-week interval. Cellular immunity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay for lymphocyte proliferation, lactate dehydrogenase release for cytotoxic T lymphocyte (CTL) activity and cytokine assay. Results: The findings demonstrated that immunization of mice with plasmid expressing HCV NS2 induced CTL response, interferon gamma production, and lymphocyte proliferation compared to negative control. The results also demonstrated that co-administration of IL-12 with the HCV NS2 plasmid induced significantly better immune response in C57BL/6 mice. Conclusion: DNA vaccine encoding HCV NS2 is an effective candidate that can trigger CTL-based immune response against HCV. In addition, the results suggested that combining the DNA vaccine approach with immune stimulatory cytokines may significantly enhance antigen-specific immune responses.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Iran. Biomed. J. [Field not mapped to EPrints] AD - Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran [Field not mapped to EPrints] AD - Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran [Field not mapped to EPrints] AD - Dept. of Microbiology, School of Medicine, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Dept. of Statistics, Gorgan ParaMedical School, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Dept. of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research Center, Khatam-Al-Anbia Hospital, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: DNA vaccine, Hepatitis C virus (HCV), IL-12, NS2 protein, 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, DNA vaccine, gamma interferon, interleukin 12, lactate dehydrogenase, nonstructural protein 2, animal experiment, article, cell activity, cell count, cell viability, cellular immunity, controlled study, cytokine production, cytotoxic T lymphocyte, DNA sequence, enzyme synthesis, female, gene expression, hepatitis C, Hepatitis C virus, immune response, immunogenicity, lymphocyte proliferation, mouse, nonhuman, NS2 gene, nucleotide sequence
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:33
Last Modified: 04 May 2015 09:57
URI: http://eprints.goums.ac.ir/id/eprint/1718

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