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Gestational diabetes induces neuronal loss in dentate gyrus in rat offspring

Kafshgiri, S.K. and Ghafari, S. and Golalipour, M.J. (2014) Gestational diabetes induces neuronal loss in dentate gyrus in rat offspring. Journal of Neurological Sciences, 31 (2). pp. 316-324. ISSN 13001817 (ISSN)

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Abstract

Background: This study was conducted to determine the effect of gestational diabetes on neuronal density in the dentate gyrus (DG) subfields of hippocampus in rats offspring. Methods: On day 1 of gestation, 10 dams randomly allocated into two control and diabetic groups. Five animals in diabetic group were received 40 mg/kg/BW of Streptozotocin (intraperitoneally) and control animals were received normal saline. Six offsprings of each gestational diabetes mellitus and controls were randomly selected at the day 7, 14 and 21. Infants were scarified and coronal sections were taken from the right dorsal hippocampus and stained with cresyl violet. The number of granular cells and thickness of layers of hippocampus in dentate gyrus lateral (DGl) and dentate gyrus media (DGm) were evaluated. Results: In P7, P14, P21, granular cells numbers of DGm were significantly reduced from (107.6±6.2, 131.6±4.6, 143.5±4.0) in controls to(84.96±2.1, 109.8±7.3, 121.05±5.6)(P<0.05) in cases, respectively and Granular cells of DGl were significantly reduced from (98.76±4.4, 125.6±4.0, 149.9±4.2) in controls to (79.98±4.2, 107.07±8.5, 117.1±6.7)(P;0.05) of cases, respectively. In DGm and DGl, the thickness of the granular and polymorph layers in P7,14 and P21 significantly decreased in gestational diabetics in comparison with controls(p<0.05). Conclusion: This study showed that the uncontrolled gestational diabetes reduces granular neurons of hippocampus in rats offspring.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English; Turkish [Field not mapped to EPrints] ST - Gestasyonel diabet yavru si{dotless}çanlari{dotless}n dentat giruslari{dotless}nda nöronal yitime yol açar [Field not mapped to EPrints] J2 - J. Neurol. Sci. [Field not mapped to EPrints] AD - Histology laboratory, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Department of Anatomical sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] AD - Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Dentate gyrus, Gestational diabetes, Granular cell, Rat, glucose, streptozocin, animal experiment, animal model, animal tissue, article, brain histology, cell density, controlled study, corona radiata (brain), dentate gyrus, dentate gyrus lateral, dentate gyrus media, dentate hilus, experimental study, female, granular cell, granular layer thickness, hippocampus, microscopy, molecular layer thickness, morphometrics, nerve cell degeneration, nonhuman, pregnancy diabetes mellitus, progeny, rat, thickness, vagina smear
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 20 Apr 2015 08:36
Last Modified: 05 May 2015 04:02
URI: http://eprints.goums.ac.ir/id/eprint/1713

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