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Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance

Mottaghi-Dastjerdi, N. and Soltany-Rezaee-Rad, M. and Sepehrizadeh, Z. and Roshandel, G. and Ebrahimifard, F. and Setayesh, N. (2014) Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance. DARU, Journal of Pharmaceutical Sciences, 22 (1). ISSN 15608115 (ISSN)

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Abstract

Background: In cancer cells, apoptosis is an important mechanism that influences the outcome of chemotherapy and the development of chemoresistance. To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues. Results: In the suppression subtractive hybridization library we constructed, the most highly overexpressed genes were humanin isoforms. Humanin is a recently identified endogenous peptide that has anti-apoptotic activity and has been selected for further study due to its potential role in the chemoresistance of gastric cancer. Upregulation of humanin isoforms was also observed in clinical samples by using quantitative real-time PCR. Among the studied isoforms, humanin isoform 3, with an expression level of 4.166 ± 1.44 fold, was the most overexpressed isoform in GC. Conclusions: The overexpression of humanin in gastric cancer suggests a role for chemoresistance and provides new insight into the biology of gastric cancer. We propose that humanin isoforms are novel targets for combating chemoresistance in gastric cancer. © 2014 Mottaghi-Dastjerdi et al.; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: Unmapped bibliographic data: C7 - 14 [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - DARU J. Pharm. Sci. [Field not mapped to EPrints] AD - Department of Pharmaceutical Biotechnology, School of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran [Field not mapped to EPrints] AD - Pharmaceutical Sciences Research Center, Sari School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Golestan, Iran [Field not mapped to EPrints] AD - Department of General Surgery, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Apoptosis, Chemoresistance, Gastric cancer, Humanin, Suppression subtractive hybridization, antineoplastic agent, complementary DNA, humanin, humanin 1, humanin 10, humanin 3, humanin 6, messenger RNA, unclassified drug, article, cancer resistance, controlled study, gene overexpression, human, human tissue, real time polymerase chain reaction, stomach cancer, suppression subtractive hybridization, upregulation
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2015 09:34
Last Modified: 09 May 2015 03:59
URI: http://eprints.goums.ac.ir/id/eprint/1710

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