Golestan University of Medical Sciences Repository

Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems

Tahamtan, A. and Ghaemi, A. and Gorji, A. and Kalhor, H.R. and Sajadian, A. and Tabarraei, A. and Moradi, A. and Atyabi, F. and Kelishadi, M. (2014) Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems. Journal of Biomedical Science, 21 (1). ISSN 10217770 (ISSN)

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Abstract

Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP NPs was efficient in CHO cells and the expression of green fluorescent proteins was well observed. In addition, NCS-DNA E7 vaccine induced the strongest E7-specific CD8+ T cell and interferon γ responses in C57BL/6 mice. Mice vaccinated with NCS-DNA E7 vaccine were able to generate potent protective and therapeutic antitumor effects against challenge with E7-expressing tumor cell line, TC-1. Conclusions: The strong therapeutic effect induced by the Chitosan-based nanodelivery suggest that nanoparticles may be an efficient carrier to improve the immunogenicity of DNA vaccination upon intramuscular administration and the platform could be further exploited as a potential cancer vaccine candidate in humans. © 2014 Tahamtan et al.

Item Type: Article
Additional Information: Unmapped bibliographic data: C7 - 69 [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - J. Biomed. Sci. [Field not mapped to EPrints] C2 - 25077570 [Field not mapped to EPrints] AD - Department of Microbiology, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Virology, School of Public Health, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Shefa Neuroscience Research CentreTehran, Iran [Field not mapped to EPrints] AD - Institut für Physiologie i, Westfälische Wilhelms-Universität Münster, Robert-Koch-StrasseMünstere, Germany [Field not mapped to EPrints] AD - Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität MünsterMünstere, Germany [Field not mapped to EPrints] AD - Department of Neurology, Westfälische Wilhelms-Universität MünsterMünstere, Germany [Field not mapped to EPrints] AD - Biochemistry Research Laboratory, Department of Chemistry, Sharif University of TechnologyTehran, Iran [Field not mapped to EPrints] AD - Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Chitosan, DNA vaccine, E7, Human papilloma virus, Tumor, chitosan, DNA vaccine, gamma interferon, interleukin 4, nanocarrier, protein E7, recombinant vaccine, Wart virus vaccine, chitosan, DNA vaccine, oncogene protein E7, Human papillomavirus type 16, protein E7, Wart virus vaccine, animal cell, animal experiment, animal model, animal tissue, antineoplastic activity, Article, cancer immunotherapy, cancer size, CD8+ T lymphocyte, cell count, CHO cell line, controlled study, cytokine production, cytokine response, cytotoxic T lymphocyte, cytotoxicity, female, genetic transfection, Human papillomavirus type 16, immune response, in vitro study, lymphocyte proliferation, mouse, nonhuman, particle size, uterine cervix cancer, zeta potential, animal, drug delivery system, human, immunology, pathology, tumor cell line, Uterine Cervical Neoplasms, vaccination, virology, Animals, Cell Line, Tumor, Chitosan, Drug Delivery Systems, Female, Humans, Mice, Papillomavirus E7 Proteins, Papillomavirus Vaccines, Uterine Cervical Neoplasms, Vaccination, Vaccines, DNA
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2015 09:40
Last Modified: 09 May 2015 06:40
URI: http://eprints.goums.ac.ir/id/eprint/1688

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