Golestan University of Medical Sciences Repository

Promoter methylation analysis of WNT/β-catenin pathway regulators and its association with expression of DNMT1 enzyme in colorectal cancer

Samaei, N.M. and Yazdani, Y. and Alizadeh-Navaei, R. and Azadeh, H. and Farazmandfar, T. (2014) Promoter methylation analysis of WNT/β-catenin pathway regulators and its association with expression of DNMT1 enzyme in colorectal cancer. Journal of Biomedical Science, 21 (1). ISSN 10217770 (ISSN)

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Abstract

Background: Aberrant DNA methylation as the most important reason making epigenetic silencing of genes is a main mechanism of gene inactivation in patients with colorectal cancer. In this study, we decided to identify promoter methylation status of ten genes encoding WNT negative regulators, and measure the expression of DNMT1 enzyme in colorectal cancer samples. Results: Aberrant methylation of APC gene was statistically significant associated with age over 50 (p = 0.017), DDK3 with male (p < 0.0001), SFRP4, WIF1, and WNT5a with increasing tumor stage (p = 0.004, p = 0.029, and p = 0.004), SFRP4 and WIF1 with tumor differentiation (p = 0.009 and p = 0.031) and SFRP2 and SFRP5 with histological type (p = 0.001 and p = 0.025). The increasing number of methylated genes correlated with the expression levels of the DNMT1 mRNA. Conclusions: The rate of gene promoter methylation of WNT pathway regulators is high in colorectal cancer cells. Hyper-methylation is associated with increased expression of the DNMT1 enzyme. © 2014 Mansour Samaei et al.; licensee BioMed Central.

Item Type: Article
Additional Information: Unmapped bibliographic data: C7 - 73 [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - J. Biomed. Sci. [Field not mapped to EPrints] C2 - 25107489 [Field not mapped to EPrints] AD - Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Molecular and Cell Biology Research Center, Mazandaran University of Medical SciencesSari, Iran [Field not mapped to EPrints] AD - Cancer Research Center, Mazandaran University of Medical SciencesSari, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Colorectal cancer, DNMT1, Promoter methylation, WNT/β-catenin signaling, beta catenin, DNA methyltransferase 1, messenger RNA, secreted frizzled related protein 2, secreted frizzled related protein 4, transcription factor Sox17, transcription factor Sox7, Wnt protein, Wnt5a protein, beta catenin, DNA, DNA (cytosine 5) methyltransferase, DNA (cytosine-5-)-methyltransferase 1, tumor protein, adult, age, Article, cancer patient, cancer staging, colorectal cancer, controlled study, DNA methylation, female, gender, human, human tissue, male, promoter region, protein expression, tumor differentiation, Wnt signaling pathway, aged, clinical trial, colorectal tumor, gene expression regulation, genetics, metabolism, middle aged, pathology, sex difference, Adult, Age Factors, Aged, beta Catenin, Colorectal Neoplasms, DNA (Cytosine-5-)-Methyltransferase, DNA Methylation, DNA, Neoplasm, Female, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Neoplasm Proteins, Promoter Regions, Genetic, Sex Factors, Wnt Signaling Pathway
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2015 09:44
Last Modified: 14 May 2015 16:28
URI: http://eprints.goums.ac.ir/id/eprint/1686

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