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A novel diblock copolymer of (monomethoxy poly [ethylene glycol]-oleate) with a small hydrophobic fraction to make stable micelles/polymersomes for curcumin delivery to cancer cells

Erfani-Moghadam, V. and Nomani, A. and Zamani, M. and Yazdani, Y. and Najafi, F. and Sadeghizadeh, M. (2014) A novel diblock copolymer of (monomethoxy poly [ethylene glycol]-oleate) with a small hydrophobic fraction to make stable micelles/polymersomes for curcumin delivery to cancer cells. International Journal of Nanomedicine, 9 (1). pp. 5541-5554. ISSN 11769114 (ISSN)

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Abstract

Curcumin is a potent natural anticancer agent, but its effectiveness is limited by properties such as very low solubility, high rate of degradation, and low rate of absorption of its hydrophobic molecules in vivo. To date, various nanocarriers have been used to improve the bioavailability of this hydrophobic biomaterial. This study investigates the encapsulation of curcumin in a novel nanostructure of monomethoxy poly(ethylene glycol)-oleate (mPEG-OA) and its anticancer effect. Tests were done to determine the critical micelle concentration (CMC), encapsulation efficiency, drug-loading efficiency, and cytotoxicity (against U87MG brain carcinoma cells and HFSF-PI3 cells as normal human fibroblasts) of some nanodevice preparations. The results of fluorescence microscopy and cell-cycle analyses indicated that the in vitro bioavailability of the encapsulated curcumin was significantly greater than that of free curcumin. Cytotoxicity evaluations showed that half maximal inhibitory concentrations of free curcumin and curcumin-loaded mPEG-OA for the U87MG cancer cell line were 48 μM and 24 μM, respectively. The Annexin-V-FLUOS assay was used to quantify the apoptotic effect of the prepared nanostructures. Apoptosis induction was observed in a dose-dependent manner after curcumin-loaded mPEG-OA treatments. Two common self-assembling structures, micelles and polymersomes, were observed by atomic force microscopy and dynamic light scat­tering, and the abundance of each structure was dependent on the concentration of the diblock copolymer. The mPEG-OA micelles had a very low CMC (13.24 μM or 0.03 g/L). Moreover, atomic force microscopy and dynamic light scattering showed that the curcumin-loaded mPEG-OA polymersomes had very stable structures, and at concentrations 1,000 times less than the CMC, at which the micelles disappear, polymersomes were the dominant structures in the dispersion with a reduced size distribution below 150 nm. Overall, the results from these tests revealed that this nanocarrier can be considered as an appropriate drug delivery system for delivering curcumin to cancer cells. © 2014 Erfani-Moghadam et al.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Int. J. Nanomed. [Field not mapped to EPrints] AD - Department of Nanobiotechnology, Tarbiat Modares UniversityTehran, Iran [Field not mapped to EPrints] AD - Department of Pharmaceutics, Zanjan University of Medical SciencesZanjan, Iran [Field not mapped to EPrints] AD - Department of Genetics, Tarbiat Modares UniversityTehran, Iran [Field not mapped to EPrints] AD - Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical SciencesGorgan, Golestan, Iran [Field not mapped to EPrints] AD - Department of Resin and Additives, Institute for Color Science and TechnologyTehran, Iran [Field not mapped to EPrints] AD - Department of Biotechnology, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Anticancer agent, Apoptosis, Bioavailability, Critical micelle concentration, Encapsulation, Nanocarrier
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 18 Apr 2015 05:21
Last Modified: 07 Sep 2016 09:51
URI: http://eprints.goums.ac.ir/id/eprint/1679

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