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Evaluating the miR-302b and miR-145 expression in formalin-fixed paraffin-embedded samples of esophageal squamous cell carcinoma

Tabrizi, M. and Khalili, M. and Vasei, M. and Nouraei, N. and Mansour Samaei, N. and Khavanin, A. and Khajehei, M. and Mowla, S.J. (2015) Evaluating the miR-302b and miR-145 expression in formalin-fixed paraffin-embedded samples of esophageal squamous cell carcinoma. Archives of Iranian Medicine, 18 (3). pp. 173-178. ISSN 10292977 (ISSN)

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Abstract

Background: MicroRNAs are involved in key cellular processes regulating, and their misregulation is linked to cancer. The miR-302-367 cluster is exclusively expressed in embryonic stem and carcinoma cells. This cluster also promotes cell reprogramming and stemness process. In contrast, miR-145 is mostly regarded as a tumor suppressor, where it regulates cellular functions such as cell division, differentiation, and apoptosis. By suppressing the main pluripotency factors (OCT4, SOX2, MYC and KLF4), miR-145 silences the self-renewal program in ESCs. Therefore, the main aim of this study is to find a potential link between the expression level of hsa-miR-302b and hsa-miR-145 with tumor vs. non-tumor as well as high-grade vs. low-grade states of the esophageal tissue samples. Methods: A total number of 40 formalin-fixed, paraffin-embedded (FFPE) samples of esophageal squamous-cell carcinoma (ESCC) were obtained, and the tumor and marginal non-tumor areas delineated and punched off by an expert pathologist. Total RNA was extracted with Trizol, and cDNA synthesized using the miRCURY LNA™ Universal RT microRNA PCR Kit. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays were performed using specific LNA-primers and SYBR Green master mix. Results: The expression level of miR-302b failed to show any significant difference, neither between tumor and their non-tumor counterparts, nor among tumors with different grades of malignancies (P > 0.05). In contrast, miR-145 was significantly down regulated in all grades of tumor samples (P < 0.001). However, its expression level could not discriminate between different grades of malignancy (P > 0.05). CONCLUSION: Our data revealed a significant down-regulation of miR-145 in ESCC tissue samples. Based on our ROC curve analysis data (AUC = 0.74, P < 0.001) miR-145 could be regarded as a potential tumor marker for diagnosis of esophageal cancer. © 2015, Academy of Medical Sciences of I.R. Iran. All rights reserved.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Arch. Iran. Med. [Field not mapped to EPrints] AD - Department of Molecular Genetics, Tarbiat Modares UniversityTehran, Iran [Field not mapped to EPrints] AD - Department of Medical Genetics and Molecular Medicine, Zanjan University of Medical SciencesZanjan, Iran [Field not mapped to EPrints] AD - Pathology laboratory, Shariati Hospital, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Human Genetics Department, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Emergency Medicine Department, Ahvaz Jundishapur University of Medical SciencesAhvaz, Iran [Field not mapped to EPrints] AD - Department of Pathology, Shiraz University of Medical SciencesShiraz, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: Esophageal cancer, FFPE, Hsa-miR-145, Hsa-miR-302b, Molecular marker, microRNA, microRNA 145, microRNA 302b, unclassified drug, Article, case control study, clinical article, diagnostic test accuracy study, esophageal squamous cell carcinoma, gene expression, human, human tissue, protein expression, real time polymerase chain reaction, receiver operating characteristic, reverse transcription polymerase chain reaction, RNA isolation, sensitivity and specificity, spectrophotometry
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 08:59
Last Modified: 15 Jan 2017 17:42
URI: http://eprints.goums.ac.ir/id/eprint/1674

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