Golestan University of Medical Sciences Repository

Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran

Rostami, S. and Torbaghan, S.S. and Dabiri, S. and Babaei, Z. and Mohammadi, M.A. and Sharbatkhori, M. and Fasihi Harandi, M. (2015) Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran. American Journal of Tropical Medicine and Hygiene, 92 (3). pp. 588-594. ISSN 00029637 (ISSN)

[img]
Preview
PDF - Published Version
Download (1MB) | Preview

Abstract

Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, presents an important medical and veterinary problem globally, including that in Iran. Different genotypes of E. granulosus have been reported from human isolates worldwide. This study identifies the genotype of the parasite responsible for human hydatidosis in three provinces of Iran using formalin-fixed paraffin-embedded tissue samples. In this study, 200 formalin-fixed paraffin-embedded tissue samples from human CE cases were collected from Alborz, Tehran, and Kerman provinces. Polymerase chain reaction amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 gene were performed for genetic characterization of the samples. Phylogenetic analysis of the isolates from this study and reference sequences of different genotypes was done using a maximum likelihood method. In total, 54.4%, 0.8%, 1%, and 40.8% of the samples were identified as the G1, G2, G3, and G6 genotypes, respectively. The findings of the current study confirm the G1 genotype (sheep strain) to be the most prevalent genotype involved in human CE cases in Iran and indicates the high prevalence of the G6 genotype with a high infectivity for humans. Furthermore, this study illustrates the first documented human CE case in Iran infected with the G2 genotype. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Am. J. Trop. Med. Hyg. [Field not mapped to EPrints] AD - Medical Laboratory of Hazrat Ali Hospital, Alborz University of Medical SciencesKaraj, Iran [Field not mapped to EPrints] AD - Laboratory Science Research Center, Golestan University of Medical SciencesGorgan, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical SciencesKerman, Iran [Field not mapped to EPrints] AD - Department of Pathology, School of Medicine, Tehran University of Medical SciencesTehran, Iran [Field not mapped to EPrints] AD - Research Center for Hydatid Disease in Iran, Afzalipour Medical Center, Kerman University of Medical SciencesKerman, Iran [Field not mapped to EPrints] AD - Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, First of Shastkila Road, Hirkan BoulevardGorgan, Golestan, Iran [Field not mapped to EPrints] AD - School of Medicine, Kerman University of Medical Sciences, Department of PathologyKerman, Iran [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled Keywords: cytochrome c oxidase, formaldehyde, paraffin, DNA sequence, echinococcosis, Echinococcus granulosus, gene sequence, genetic analysis, genotype, human, human tissue, Iran, nonhuman, nucleotide sequence, parasite isolation, phylogeny, polymerase chain reaction, prevalence, ram (sheep), Review, Echinococcus granulosus, Ovis aries
Subjects: مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
موارد کلی
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Apr 2015 08:52
Last Modified: 09 Jan 2017 07:34
URI: http://eprints.goums.ac.ir/id/eprint/1659

Actions (login required)

View Item View Item