Golestan University of Medical Sciences Repository

Genotyping and sequence characterization of the NSP4 gene of human group A rotavirus strains in Northern Iran

Khalkhali, P. and Khavandegar, A. and Mozhgani, S.-H. and Teimoori, A. and Moradi, A. and Ajorloo, M. and Lorestani, N. and Kaveh, K. and Akbar, S. and Razavi Nikoo, H. (2021) Genotyping and sequence characterization of the NSP4 gene of human group A rotavirus strains in Northern Iran. Journal of Medical Virology, 93 (8). pp. 4824-4830.

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Rotavirus is known to be responsible for remarkable numbers of severe diarrheal episodes and even death in infants and young children. In this study, we aimed to survey genetic diversity and variation analysis of viroporin, which is encoded by the rotavirus NSP4 segment. Thirty-five rotavirus-positive specimens were obtained, and RNA extraction and polymerase chain reaction amplification were performed. After the sequencing process, four specimens were excluded, and the final 31 samples remained for genetic diversity and variation analysis. The predominant single G/P combination was G1P8 (~78%), followed by G2P8 (~13%), and equal percentages (3%) of G2P4, G3P8, and G-non-typeable-P8. Further analyses revealed that variations could be found in the three regions of NSP4, including VP4 binding site (aa 112�146), double-layered particle binding site (aa 161�175), and finally, in the predicted amphipathic alpha-helix. Phylogenic tree analysis demonstrated that the mentioned samples clustered with genotype E1 and E2 reference sequences. As previously reported in the literature, in this study, it was revealed that no apparent correlation exists in the deduced amino acid sequences corresponding to this region between the rotaviruses collected from patients with and without diarrhea. © 2021 Wiley Periodicals LLC

Item Type: Article
Additional Information: cited By 0
Subjects: آسیب شناسی QZ
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 03 Jul 2021 10:28
Last Modified: 03 Jul 2021 10:28
URI: http://eprints.goums.ac.ir/id/eprint/11117

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