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Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line

Mohebbi, A. and Azadi, F. and Hashemi, M.M. and Askari, F.S. and Razzaghi, N. (2021) Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line. Intervirology, 64 (1). pp. 22-26.

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Abstract

Background: Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect. Objective: Use of root extract of Havachoobe (Onosma dichroanthum BoissI), a Northern Iranian native medical herb, for assessment of its anti-HBsAg secretion activity. Methods: Havachoobe had been bought at a nearby apothecary store. Plant root extract was obtained using a hydroalcoholic process. Cytotoxic activity of the extract was examined on PLC/PRF/5 cells using MTT assay. ELISA has been used to measure HBsAg in the treated cell line supernatants. In addition, real-time PCR analysis was performed to evaluate the expression of HBsAg before and after treatment of Onosma in vitro. Results: The results showed very low root extract cytotoxicity at concentrations under 8 μg/mL. Tissue culture infectious dose 50 was obtained at 63.78 μg/mL. In a dose-dependent and time-dependent manner, a significantly reduced HBsAg secretion was observed at a concentration of 8 ppm at 12 h post-treatment. The real-time PCR result showed relative decreased HBsAg expression at all doses at 12 h post-treatment time. Discussion: In this study, we first reported anti-HBsAg activity on an Iranian herbal medicine. Havachoobe root extract was shown to be able to inhibit HBsAg in a dose-dependent and time-dependent manner. We find the extract exerts its inhibitory effect of HBsAg by targeting transcription of HBsAg. © 2021 S. Karger AG. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: antivirus agent; hepatitis B surface antigen; Onosma dichroanthum boissi extract; plant extract; unclassified drug, Article; Boraginaceae; controlled study; dose response; drug cytotoxicity; gene expression level; Hepatitis B virus; herb; human; human cell; ID50; in vitro study; MTT assay; nonhuman; Onosma dichroanthum boissi; plant root; PLC/PRF/5 cell line; priority journal; qualitative analysis; real time polymerase chain reaction; sandwich ELISA; tcid50; virus release
Subjects: سیستم گوارشی WI
بیماریهای واگیرWC
فارماکولوژی QV > فارماکوگنوزی
> دانشکده داروسازی > فارماکولوژی QV > فارماکوگنوزی

میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 23 Feb 2021 07:39
Last Modified: 23 Feb 2021 07:39
URI: http://eprints.goums.ac.ir/id/eprint/10959

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