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Association of grp78, hif-1α and bag3 expression with the severity of chronic lymphocytic leukemia

Ijabi, R. and Roozehdar, P. and Afrisham, R. and Moradi-Sardareh, H. and Kaviani, S. and Ijabi, J. and Sahebkar, A. (2020) Association of grp78, hif-1α and bag3 expression with the severity of chronic lymphocytic leukemia. Anti-Cancer Agents in Medicinal Chemistry, 20 (4). pp. 429-436.

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Abstract

Introduction: Parallel with the progression of Chronic Lymphocytic Leukemia (CLL), the levels of 78KDa Glucose-Regulated Protein (GRP78) and Hypoxia-Inducible Factor 1 alpha (HIF-1α) are increased as they may activate the induction of anti-apoptotic proteins such as BCL2 Associated Athanogene 3 (BAG3). Previous studies have indicated that there is a positive correlation among GRP78, HIF-1α and BAG3. Objective: This study aimed to evaluate the effect of metabolic factors involved in invasive CLL on apoptotic factors. Methods: A case-control study was conducted on 77 patients diagnosed with CLL along with 100 healthy individuals. Cell blood count was performed for all participants. According to Binet's classification, CLL patients were divided into different groups. B cells were isolated from the peripheral blood of CLL patients by binding to anti-CD19 beads. The expression of BAG3, GRP78 and HIF-1α genes was analyzed using the RT-PCR method. To confirm the results of RT-PCR, western blot analysis was carried out. Results: The results showed that there was a strong association among the expression of BAG3, GRP78 and HIF-1α. The stage of CLL in patients was highly correlated with the expression rate of each gene (p<0.001). Accordingly, the western blot analysis indicated that the concentrations of GRP78 and HIF-1α were significantly higher than the expression of BAG3, considering the stage of CLL. Conclusion: It was shown that increased expression of GRP78 and HIF-1α could result in the elevation of BAG3, as well as the disease progression. Therefore, the role of these metabolic factors might be more pronounced compared with the anti-apoptotic agents to monitor disease progression in CLL patients. © 2020 Bentham Science Publishers.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: actin; Bcl2 associated athanogene 3; glucose regulated protein 78; hypoxia inducible factor 1alpha; protein bcl 2; unclassified drug, adult; aged; Article; autoradiography; B lymphocyte; blood cell count; cancer staging; case control study; cell isolation; chronic lymphatic leukemia; controlled study; densitometry; disease exacerbation; disease severity; electrochemiluminescence; gene expression level; human; human cell; immunoblotting; major clinical study; peripheral blood mononuclear cell; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; very elderly; Western blotting
Subjects: سیستم های خونی و لنفاوی WH
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Jun 2020 06:48
Last Modified: 15 Jun 2020 06:48
URI: http://eprints.goums.ac.ir/id/eprint/10683

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