Golestan University of Medical Sciences Repository

Comparison of two molecular diagnostic methods for identifying beijing genotype of mycobacterium tuberculosis

Mahghani, G.A. and Kargar, M. and Kafilzadeh, F. and Davoodi, H. and Ghaemi, E.A. (2020) Comparison of two molecular diagnostic methods for identifying beijing genotype of mycobacterium tuberculosis. Iranian Journal of Microbiology, 12 (3). pp. 209-215.

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Abstract

Background and Objectives: The Beijing family of Mycobacterium tuberculosis has been identified as a severe pathogen among this species and found in many clinical isolates during the last decade. Early identification of such genotype is important for better prevention and treatment of tuberculosis. The present study performed to compare the efficiency of Real-Time PCR and IS6110-Based Inverse PCR methods to identify the Beijing family. Materials and Methods: This study was carried out on 173 clinical isolates of Mycobacterium tuberculosis complex in Golestan Province, northern Iran. DNA extraction performed by boiling and determining the Beijing and non-Beijing strains carried out using Real-Time PCR and IS6110-Based Inverse PCR. Results: In both Real-Time PCR and IS6110-Based Inverse PCR method, 24 specimens (13.9) of the Beijing family were identified and the result of the IS6110-Based Inverse PCR method showed that all the Beijing strains in this region belonged to the Ancient Beijing sub-lineage. Conclusion: Although the efficacy of the two methods in the diagnosis of the Beijing family is similar, the IS6110-Based Inverse PCR is more applicable to the ability to detect new and old Beijing family. © 2020, Tehran University of Medical Science. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: article; China; controlled study; DNA extraction; genotype; human; intermethod comparison; inverse polymerase chain reaction; Iran; molecular diagnosis; Mycobacterium tuberculosis complex; nonhuman; real time polymerase chain reaction
Subjects: سیستم تنفسی WF
بیماریهای واگیرWC
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Jun 2020 08:08
Last Modified: 10 Jun 2020 08:08
URI: http://eprints.goums.ac.ir/id/eprint/10665

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