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Immune responses modulation by curcumin and allergen encapsulated into PLGA nanoparticles in mice model of rhinitis allergic through sublingual immunotherapy

Shahgordi, S. and Sankian, M. and Yazdani, Y. and Mashayekhi, K. and Hasan Ayati, S. and Sadeghi, M. and Saeidi, M. and Hashemi, M. (2020) Immune responses modulation by curcumin and allergen encapsulated into PLGA nanoparticles in mice model of rhinitis allergic through sublingual immunotherapy. International Immunopharmacology, 84.

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Abstract

The purpose of this study was the combination of curcumin and ovalbumin in free form or encapsulated into PLGA NPs (polylactic co-glycolic acid nanoparticles) to enhance their sublingual immunotherapy (SLIT) efficiency in mouse model of rhinitis allergic. PLGA NPs containing curcumin (CUR), ovalbumin (OVA) or both were prepared by emulsion-solvent evaporation method and characterized. After sensitization of BALB/C mice with ovalbumin, SLIT with free or encapsulated formulations was carried out and immunological profiles were evaluated. SLIT treatment with all synthesized PLGA formulations lead to significantly decreased total IgE. The combination immunotherapy in the present of free form of curcumin or ovalbumin with encapsulated forms of the another substance (P.OVA-CUR 10 and P.CUR 5-OVA), showed the highest level of IFN-γ:IL-4 compared to other target groups. On the other hands, a significant increasment was observed in this ratio between these optimal groups and treated group with subcutaneous administration of OVA as the most commonly used method for immunotherapy. The study of nasal lavage fluid (NALF) showed significant decreased levels of total and eosinophil cell count in the traeted nano-formulation groups. The histopathological results of NAL were also like normal with no cellular infiltration and no inflammation in the optimal formulations. Therefore, using curcumin and nanoparticles with allergen can be considerd as potential immune modulatory agents. © 2020 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: curcumin; gamma interferon; immunoglobulin E; immunoglobulin E antibody; interleukin 4; nanoparticle; ovalbumin; polyglactin, allergic rhinitis; animal experiment; animal model; animal tissue; Article; atomic force microscopy; controlled study; cytokine production; cytokine release; emulsion; eosinophil count; female; histopathology; immune response; immunomodulation; lung parenchyma; mouse; nanoencapsulation; nasal lavage fluid; nonhuman; nose mucosa; particle size; priority journal; sensitization; spleen cell; sublingual immunotherapy; zeta potential
Subjects: بیماریهای گوش وحلق وبینی WV
بیماریهای تغذیه ومتابولیکی WD
QU بیوشیمی
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 16 May 2020 08:42
Last Modified: 16 May 2020 08:42
URI: http://eprints.goums.ac.ir/id/eprint/10621

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