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Fluorescence detection of laccases activity by the photoinduced electron transfer (PET) process

Arabi, M.S. and Karami, C. and Taher, M.A. and Ahmadi, E. (2020) Fluorescence detection of laccases activity by the photoinduced electron transfer (PET) process. Journal of Biological Inorganic Chemistry, 25 (1). pp. 151-159.

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Abstract

Abstract: Laccases play a vital role in some physiological processes, for example in morphogenesis, carbon cycle, and defense against parasitism. So, designing a high-sensitivity accurate method is essential for researchers. In this study, a simple fluorescence method based on the function of carbon nitride (g-C3N4) by dopamine is synthesized. For the design of this sensor, carbon nitride (g-C3N4) is initially synthesis by using a simple method, which is carried out by heating melamine at 550 °C for 3 h and modifying it with dopamine by a linker such as glutaraldehyde. However, the g-C3N4�Dopa produced by this method, with an excitation wavelength of 330 nm, has a fluorescence emission at 466 nm. When laccase and g-C3N4�Dopa were mixed, dopamine with redox property was oxidized to dopaquinone; this causes the phenomenon of photoinduced electron transfer (PET) process between g-C3N4 and the dopaquinone. Hence, fluorescence quenching occurs due to this phenomenon. As a result of these discussions, a sensor for the laccase activity was designed based on the fluorescence quenching degree, supporting a linear range of 0.0�400.0 U L�1 with the detection limit of 2.0 U L�1. Using this sensor, the activity of the laccase enzyme in the human serum samples is measured. Graphic abstract: Dopamine-functionalized carbon nitride was prepared and utilized for the highly sensitive detection of laccases activity.Figure not available: see fulltext. © 2019, Society for Biological Inorganic Chemistry (SBIC).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: carbon; carbon nitride dopa; dopamine; dopamine derivative; dopaquinone; glutaraldehyde; laccase; melamine; nitrogen derivative; quinone derivative; unclassified drug, Article; chemical phenomena; chemical reaction; chemical structure; controlled study; enzyme activity; enzyme analysis; fluorescence; fluorescence analysis; heating; oxidation; oxidation reduction state; photochemical quenching; photoinduced electron transfer; priority journal; synthesis
Subjects: QTفیزیولوژی
QU بیوشیمی
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 22 Apr 2020 06:06
Last Modified: 22 Apr 2020 06:06
URI: http://eprints.goums.ac.ir/id/eprint/10599

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