Golestan University of Medical Sciences Repository

Antifungal activity of ZnO nanoparticles and nystatin and downregulation of SAP1-3 genes expression in fluconazole-resistant candida albicans isolates from vulvovaginal candidiasis

Sedigheh Hosseini, S. and Joshaghani, H. and Shokohi, T. and Ahmadi, A. and Mehrbakhsh, Z. (2020) Antifungal activity of ZnO nanoparticles and nystatin and downregulation of SAP1-3 genes expression in fluconazole-resistant candida albicans isolates from vulvovaginal candidiasis. Infection and Drug Resistance, 13. pp. 385-394.

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Abstract

Purpose: Antifungal resistance and virulence properties of Candida albicans (C. albicans) are growing health problems worldwide. The present study aims to investigate the effect of Zinc Oxide (ZnO) nanoparticles and Nystatin on SAP1-3 genes expression in C. albicans isolates of females with Vulvovaginal Candidiasis (VVC) isolated from Sayad Shirazi Obstetrics and Gynecology Hospital in Northeastern Iran during 2017�2018. Patients and Methods: In this descriptive-analytic study, vaginal samples were collected from 280 VVC women. 196 (70) of C. albicans isolates were identified by phenotypic and ITS genotypic methods. Susceptibility to Fluconazole C. albicans isolates was determined by the disk diffusion method. Detection of ERG11 gene was done by RT-PCR technique. Results: It was revealed that PCR amplified the ERG11 gene in all of the Fluconazole-resistant isolates. Real-time PCR was used to survey the effects of 3±1.7µg/mL concentrations of ZnO nanoparticles and Nystatin on expression of SAP1-3 genes before and after treatment. 186 (95) susceptible C. albicans and 10 (5) Fluconazole-resistant C. albicans isolates from VVC were exposed to sub-minimum inhibitory concentrations (Sub-MIC) of ZnO-np (range=0.02�12 μg/ mL). Sub-MIC concentration was used for each strain, which reduced the expression of SAP1-3 genes to 1.8 MIC in the vaginal swabs. The observed reduction in gene expression was significant for both ZnO nanoparticles and Nystatin (P=0.01 and P=0.07, respectively). Conclusion: ZnO as antifungal agent can well reduce the growth and gene expression of SAP1-3 in the pathogenesis of VVC. © 2020 Hosseini et al.

Item Type: Article
Additional Information: cited By 0
Subjects: بیماریهای زنان WP
QTفیزیولوژی
QU بیوشیمی
فارماکولوژی QV
> دانشکده داروسازی > فارماکولوژی QV

میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2020 06:11
Last Modified: 14 Apr 2020 06:11
URI: http://eprints.goums.ac.ir/id/eprint/10526

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