Golestan University of Medical Sciences Repository

Efficient co-cultivation of human fibroblast cells (HFCs) and adipose-derived stem cells (ADSs) on gelatin/PLCL nanofiber

Ranjbar-Mohammadi, M. and Mousavi, E. and Hashemi, M.M. and Abbasian, M. and Asadi, J. and Esmaili, E. and Fesharaki, M. and Asadi, P. and Arab-Bafrani, Z. (2020) Efficient co-cultivation of human fibroblast cells (HFCs) and adipose-derived stem cells (ADSs) on gelatin/PLCL nanofiber. IET Nanobiotechnology, 14 (1). pp. 73-77.

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In this study, we investigated whether the nanofibers produced by natural-synthetic polymers can probably promote the proliferation of co-cultured adipose-derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L-lactide co-ϵ-caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real-time polymerase chain reaction were applied to assess the proliferation of co-cultured ADSs/ HFCs and the collagen type I and III synthesis, respectively. The concentrations of two cytokines including fibroblast growth factor-basic and transforming growth factor-β1 were also measured in culture medium of co-cultured ADSs/HDCs using enzyme-linked immunosorbent assay assay. Actually, nanofibers exhibited proper structural properties in terms of stability in cell proliferation and toxicity analysis processes. Gel/PLCL nanofiber promoted the growth and the adhesion of HFCs. Our results showed in contact co-culture of ADSs/HFCs on the Gel/PLCL nanofiber increased cellular adhesion and proliferation synergistically compared to non-coated plate. Also, synthesis of collagen and cytokines secretion of co-cultured ADSs/HFCs on Gel/PLCL scaffolds is significantly higher than non-coated plates. To conclude, the results suggest that Gel/PLCL nanofiber can imitate physiological characteristics in vivo and enhance the efficacy of co-cultured ADSs/HFCs in wound healing process. © The Institution of Engineering and Technology 2019.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Adhesion; Blending; Cell culture; Cell proliferation; Collagen; Fibroblasts; Fluorocarbons; Plastic coatings; Plates (structural components); Polymerase chain reaction; Stem cells, Adipose derived stem cells; Enzyme linked immunosorbent assay; Fibroblast growth factor; Fluorescent microscopy; Human fibroblast cells; Physiological characteristics; Quantitative real-time polymerase chain reaction; Transforming growth factors, Nanofibers
Subjects: QTفیزیولوژی
QU بیوشیمی
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 14 Apr 2020 06:34
Last Modified: 14 Apr 2020 06:34
URI: http://eprints.goums.ac.ir/id/eprint/10516

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