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A comparative evaluation of regulatory T cells profile among acute and chronic cutaneous leishmaniasis using flow cytometry

Firouzjaie-Karder, F. and Akhoundi, B. and Mohebali, M. and Ardalan, F.A. and Rahimi-Foroushani, A. and Mesgarian, F. and Hajjaran, H. and Mortazavi, H. and Shakiba, Y. and Charehdar, S. and Elikaee, S. and Kakooei, Z. and Shafeghat, Z. (2019) A comparative evaluation of regulatory T cells profile among acute and chronic cutaneous leishmaniasis using flow cytometry. Iranian Journal of Parasitology, 14 (2). pp. 190-196.

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Background: Cutaneous leishmaniasis (CL) is described as a major health problem in many countries of the world. Regulatory T cells (Tregs) are characterized as one of immunologic indexes. One of the best methods to determine of Tregs percentage is flow cytometry. The aim of this study was determination of the role of Tregs profile among acute and chronic forms of human CL using flow cytometry analysis. Methods: This study was conducted on 24 patients referred to Laboratory of Leishmaniasis, Tehran University of Medical Sciences, Tehran, Iran with acute and 14 patients with chronic phases of CL as well as 15 healthy individuals as control group in 2015-2016. After microscopic examination, 2 ml of peripheral blood samples were collected for determining percentage of CD4 + CD25+ CD127 low Tregs by using flow cytometry method. Results: Using flow cytometry analysis, the average percentage of Tregs were calculated 5.73, 6.71 and 6.61 for acute, chronic and healthy individuals, respectively. With SPSS software and Scheffe multiple comparison tests, the differences within in these groups are statistically significant (P=0.04) and between the acute and chronic group, there was marginally significant with approximately 91 of confidence level (P=0.088). Conclusion: Marginally differences were found significantly among averages of Regulatory T cells, acute and chronic phases of CL. Further comprehensive studies can be needed to verify the role of Tregs in both phases of CL cases. © 2019, Tehran University of Medical Sciences (TUMS). All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: CD4 antigen; cytotoxic T lymphocyte antigen 4; gamma interferon; glucocorticoid induced tumor necrosis factor receptor; interleukin 10; interleukin 2 receptor alpha; interleukin 4; interleukin 7 receptor; meglumine antimonate; transcription factor FOXP3, adolescent; adult; aged; Article; blood sampling; child; clinical article; controlled study; female; flow cytometry; human; immune response; immunosuppressive treatment; male; parasitology; protein expression; regulatory T lymphocyte; skin leishmaniasis
Subjects: بیماریهای واگیرWC
بیماریهای پوست WR
انگل شناسی QX
میکروب شناسی وایمنی شناسی QW
بهداشت عمومی WA
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Dec 2019 10:12
Last Modified: 25 Dec 2019 10:12
URI: http://eprints.goums.ac.ir/id/eprint/10482

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