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Myeloid cell leukemia-1 (MCL-1) siRNA therapy showed cytotoxic effect on T cells acute lymphoblastic leukemia

Askarian-Amiri, S. and Aval, F.O. and Azadmehr, A. and Oladnabi, M. and Vesiehsari, M.J. and Hajiahmadi, M. (2019) Myeloid cell leukemia-1 (MCL-1) siRNA therapy showed cytotoxic effect on T cells acute lymphoblastic leukemia. International Journal of Cancer Management, 12 (4).

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Abstract

Background: T-lineage acute lymphoblastic leukemia (T-ALL) is a malignant hyperplastic disease of the hematopoietic system. This tumor is the most common tumor in children and adolescents. Myeloid cell leukemia-1 (Mcl-1) is described as a prosurvival protein from the Bcl2 family. It is an important factor in routine cancer treatments. In fact, in different types of cancers, Mcl-1 downregulation can be a potential target. Objectives: The present study aims to evaluate the cytotoxic effect of MCL-1 siRNA in T-ALL cells. Methods: The present study evaluated the effects of Mcl-1 small interfering RNAs (siRNAs) on survival in Jurkat cells. Specific Mcl-1 siRNA was transfected and using quantitative real-time PCR, the relative expression of Mcl-1 mRNA was determined. Moreover, cell survival was determined using the colorimetric MTT assay. Results: The expression of mRNA reduced effectively in a dose-dependent manner at 48 hours after transfection with Mcl-1 siRNA. In addition, Mcl-1 siRNA treatment could significantly reduce tumor cell survival. Conclusions: Based on the results, downregulation of Mcl-1 by specific siRNAs in T-ALL cells can effectively reduce cell survival. Therefore, Mcl-1 siRNA may be a complementary agent along with standard methods in the treatment of T-ALL. © 2019, Author(s).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: beta actin; protein bcl 2; protein mcl 1; protein mcl 1 small interfering RNA; small interfering RNA; unclassified drug, acute lymphoblastic leukemia; Article; cell survival; controlled study; cytotoxic T lymphocyte; cytotoxicity; gene expression; gene repression; gene therapy; genetic transfection; human; human cell; Jurkat cell line; mRNA expression level; MTT assay; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA isolation
Subjects: سیستم های خونی و لنفاوی WH
آسیب شناسی QZ
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 25 Dec 2019 10:25
Last Modified: 25 Dec 2019 10:25
URI: http://eprints.goums.ac.ir/id/eprint/10481

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